Expression analysis onto microarrays of randomly selected cDNA clones highlights HOXB13 as a marker of human prostate cancer

Edwards, S., Campbell, C., Flohr, P., Shipley, J., Giddings, I., Te-Poele, R., Dodson, A., Foster, C., Clark, J., Jhavar, S., Kovacs, G. and Cooper, C. S. ORCID: https://orcid.org/0000-0003-2013-8042 (2004) Expression analysis onto microarrays of randomly selected cDNA clones highlights HOXB13 as a marker of human prostate cancer. British Journal of Cancer, 92 (2). pp. 376-381. ISSN 0007-0920

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Abstract

In a strategy aimed at identifying novel markers of human prostate cancer, we performed expression analysis using microarrays of clones randomly selected from a cDNA library prepared from the LNCaP prostate cancer cell line. Comparisons of expression profiles in primary human prostate cancer adjacent normal prostate tissue, and a selection of other (nonprostate) normal human tissues, led to the identification of a set of clones that were judged as the best candidate markers of normal and/or malignant prostate tissue, DNA sequencing of the selected clones revealed that they included 10 genes that had previously been established as prostate markers: NKX3.1, KLK2, KLK3 (PSA), FOLH1 (PSMA), STEAP2, PSGR, PRAC, RDH11, Prostein and FASN. Following analysis of the expression patterns of all selected and sequenced genes through interrogation of SAGE databases, a further three genes from our clone set, HOXB13, SPON2 and NCAM2, emerged as additional candidate markers of human prostate cancer. Quantitative RT-PCR demonstrated the specificity of expression of HOXB13 in prostate tissue and revealed its ubiquitous expression in a series of 37 primary prostate cancers and 20 normal prostates. These results demonstrate the utility of this expression-microarray approach in hunting for new markers of individual human cancer types.

Item Type: Article
Additional Information: Funding Information: This work is supported by the National Cancer Research Institute, Cancer Research UK, the Medical Research Council and The Rosetrees Trust. We thank Christine Bell for typing this manuscript and Dr Andy Norman for carrying out statistical analyses.
Uncontrolled Keywords: expression,hoxb13,microarray,prostate,oncology,cancer research,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2730
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 18 Jul 2022 12:30
Last Modified: 12 Aug 2022 05:42
URI: https://ueaeprints.uea.ac.uk/id/eprint/86468
DOI: 10.1038/sj.bjc.6602261

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