Construction of tissue microarrays from prostate needle biopsy specimens

Jhavar, S., Corbishley, C. M., Dearnaley, D., Fisher, C., Falconer, A., Parker, C., Eeles, R. and Cooper, C. S. ORCID: https://orcid.org/0000-0003-2013-8042 (2005) Construction of tissue microarrays from prostate needle biopsy specimens. British Journal of Cancer, 93 (4). pp. 478-482. ISSN 0007-0920

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Abstract

Needle biopsies are taken as standard diagnostic specimens for many cancers, but no technique exists for the high-throughput analysis of multiple individual immunohistochemical (IHC) markers using these samples. Here we present a simple and highly reliable technique for constructing tissue microarrays (TMAs) from prostatic needle biopsies. Serial sectioning of the TMAs, called 'Checkerboard TMAs', facilitated expression analysis of multiple proteins using IHC markers. In total, 100% of the analysed biopsies within the TMA both preserved their antigenicity and maintained their morphology. Checkerboard TMAs will allow the use of needle biopsies (i) alongside other tissue specimens (trans-urethral resection of prostates and prostatectomies in the case of prostate cancer) in clinical correlation studies when searching for new prognostic markers, and (ii) in a diagnostic context for assessing expression of multiple proteins in cancers from patients prior to treatment.

Item Type: Article
Additional Information: Funding Information: We are grateful to Sue Clinton for her initial help in cutting the TMA blocks, Dr Nasiri for his help in initial reviewing of the biopsy slides, David Hudson and Sian Rizzo for their help in taking photomicrographs of the TMA sections and Anne Fletcher for providing a slide of conventional TMA to photograph the section of a conventional TMA core. This study was funded by (i) The National Cancer Research Institute (NCRI), (ii) The Rosetrees Trust and (iii) The Grand Charity of Freemasons. This work was undertaken in The Royal Marsden NHS Trust, which received a proportion of its funding from the NHS Executive; the views expressed in this publication are ours, and not necessarily those of the NHS Executive. This work was supported by the Institute of Cancer Research, the Bob Champion Cancer Trust and Cancer Research UK Section of Radiotherapy CUK grant number C46/ A2131.
Uncontrolled Keywords: cancer,immunohistochemistry,needle biopsy,prostate,tissue microarray,oncology,cancer research,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2730
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 18 Jul 2022 12:30
Last Modified: 12 Aug 2022 05:42
URI: https://ueaeprints.uea.ac.uk/id/eprint/86466
DOI: 10.1038/sj.bjc.6602726

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