Mir143 expression inversely correlates with nuclear ERK5 immunoreactivity in clinical prostate cancer

Ahmad, I., Singh, L. B., Yang, Z. H., Kalna, G., Fleming, J., Fisher, G., Cooper, C. ORCID: https://orcid.org/0000-0003-2013-8042, Cuzick, J., Berney, D. M., Møller, H., Scardino, P. and Leung, H. Y. (2013) Mir143 expression inversely correlates with nuclear ERK5 immunoreactivity in clinical prostate cancer. British Journal of Cancer, 108 (1). pp. 149-154. ISSN 0007-0920

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Abstract

* Background: Aberrant mitogen/extracellular signal-regulated kinase 5 (MEK5)-extracellular signal-regulated protein kinase 5 (ERK5)-mediated signalling has been implicated in a number of tumour types including prostate cancer (CaP). The mechanism for ERK5 activation in CaP remains to be fully elucidated. Studies have recently implicated the role of microRNA (miRNA) mir143 expression in the regulation of ERK5 expression. * Methods: We utilised a tissue microarray (TMA) of 530 CaP cores from 168 individual patients and stained for both mir143 and ERK5. These TMAs were scored by a combination of observer and automated methods. * Results:We observed a strong inverse relation between ERK5 and mir143, which manifested itself most strongly in the subgroup of 417 cores with non-zero mir143 and ERK5 immunoreactivity, or with only one of mir143 or ERK5 being zero (cc=0.2558 and P<0.0001). Mir143 neither correlate with Gleason scores or prostate-specific antigen levels, nor was it a predictor of disease-specific survival on univariate analysis. * Conclusion:Although the mechanism for ERK5 activation in CaP remains to be fully elucidated, we have further validated the potential role of mir143 in regulating ERK5 levels in the clinical context. In addition, we demonstrate that the automated counting method for nuclear ERK5 is a clinically useful alterative to observer counting method in patient stratification in the context of ERK5 targeting therapy.

Item Type: Article
Additional Information: Funding Information: Cancer Research UK funded research work in the Urology Group, Beatson Institute for Cancer Research. IA was funded by a clinical research fellowship from the Medical Research Council and Cancer Research UK. We thank Clare Orange for technical assistance, and the ‘Think Pink’ charity for the purchase of the slide scanner.
Uncontrolled Keywords: erk5,mir143,prostate cancer,oncology,cancer research,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2730
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 18 Jul 2022 11:30
Last Modified: 12 Aug 2022 05:41
URI: https://ueaeprints.uea.ac.uk/id/eprint/86451
DOI: 10.1038/bjc.2012.510

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