Tenascin-C inhibits oligodendrocyte precursor cell migration by both adhesion-dependent and adhesion-independent mechanisms

Kiernan, B. W., Götz, Bernhard, Faissner, Andreas and Ffrench-Constant, Charles ORCID: https://orcid.org/0000-0002-5621-3377 (1996) Tenascin-C inhibits oligodendrocyte precursor cell migration by both adhesion-dependent and adhesion-independent mechanisms. Molecular and Cellular Neurosciences, 7 (4). pp. 322-335. ISSN 1044-7431

Full text not available from this repository. (Request a copy)

Abstract

Tenascin-C is present within the developing central nervous system during oligodendrocyte precursor cell migration. Tenascin-C is antiadhesive for oligodendrocytes, suggesting a role in controlling the migration of oligodendrocyte precursors and hence the pattern of myelination. Here we show directly that tenascin-C is a repulsive (or antiadhesive) substrate for primary oligodendrocyte precursors and also inhibits their migration. The antimigratory effect of tenascin-C on oligodendroglia is mediated through two distinct mechanisms; reduced substrate adhesion and a direct inhibition of cell migration that is independent of adhesion. These two effects map to different domains of the tenascin-C molecule. The repulsive effect maps to the EGF-like repeats and the alternatively spliced FN III repeats while the direct migration-inhibiting effect maps to FN III repeats 7-8. Our results show tenascin-C to have the novel property of inhibiting migration by both adhesion-dependent and adhesion-independent mechanisms, with different regions of the same molecule responsible for the two effects.

Item Type: Article
Additional Information: Funding Information: We are grateful to R. Keynes for comments on the manuscript. Funds were provided by the Wellcome Trust (C.ff-C.), German Research Council (DFG, SFB 317/A2 to A.F. and B.G.), and the Science and Engineering Research Council (B.W.K.). A.F. is a recipient of a Schilling-professorship for neuroscience and C.ff-C. is a Wellcome Trust senior clinical fellow.
Uncontrolled Keywords: molecular biology,cellular and molecular neuroscience,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 16 Jul 2022 16:30
Last Modified: 24 Sep 2022 19:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/86384
DOI: 10.1006/mcne.1996.0024

Actions (login required)

View Item View Item