Expression of the rat homologue of the Drosophila fat tumour suppressor gene

Ponassi, Marco, Jacques, Thomas S., Ciani, Lorenza and Ffrench-Constant, Charles ORCID: (1999) Expression of the rat homologue of the Drosophila fat tumour suppressor gene. Mechanisms of Development, 80 (2). pp. 207-212. ISSN 0925-4773

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We have sequenced and defined the expression during rat embryogenesis of the protocadherin fat, the murine homologue of a Drosophila tumour suppressor gene. As previously described for human fat, the sequence encodes a large protocadherin with 34 cadherin repeats, five epidermal growth factor (EGF)- like repeats containing a single laminin A-G domain and a putative transmembrane portion followed by a cytoplasmic sequence. This cytoplasmic sequence shows homology to the β-catenin binding regions of classical cadherin cytoplasmic tails and also ends with a PDZ domain-binding motif. In situ hybridization studies at E15 show that fat is predominately expressed in fetal epithelial cell layers and in the CNS, although expression is also seen in tongue musculature and condensing cartilage. Within the CNS, expression is seen in the germinal regions and in areas of developing cortex, and this neural expression pattern is also seen at later embryonic (E18) and postnatal stages. No labelling was seen in adult tissues except in the CNS, where the remnant of the germinal zones, as well as the dentate gyrus, continue to express fat.

Item Type: Article
Additional Information: Funding Information: This work was funded by the Multiple Sclerosis Society of Great Britain and Northern Ireland and the Cancer Research Campaign, and also by a Human Capital and Mobility Award to M.P. and a Wellcome Trust Prize Studentship to T.J. We are grateful to Monique Jouet, Emma Frost, Kathi Blaschuk, Philip Buttery, Luciano Zardi and Roberto Gherzi for their comments on the manuscript. An erratum is available which corrects an author affiliation address: The content of the article is not affected.
Uncontrolled Keywords: cadherin,egf repeat,embryo,epithelium,fat,laminin a-g domain,neural stem cell,neuroepithelium,neurosphere,pdz domain,protocadherin,subventricular zone,tumour suppressor,ventricular zone,embryology,developmental biology ,/dk/atira/pure/subjectarea/asjc/2700/2710
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 16 Jul 2022 13:30
Last Modified: 22 Oct 2022 18:38
DOI: 10.1016/S0925-4773(98)00217-2

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