Laminin enhances the growth of human neural stem cells in defined culture media

Hall, Peter E., Lathia, Justin D., Caldwell, Maeve A. and Ffrench-Constant, Charles ORCID: (2008) Laminin enhances the growth of human neural stem cells in defined culture media. BMC Neuroscience, 9. ISSN 1471-2202

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* Background: Human neural stem cells (hNSC) have the potential to provide novel cell-based therapies for neurodegenerative conditions such as multiple sclerosis and Parkinson's disease. In order to realise this goal, protocols need to be developed that allow for large quantities of hNSC to be cultured efficiently. As such, it is important to identify factors which enhance the growth of hNSC. In vivo, stem cells reside in distinct microenvironments or niches that are responsible for the maintenance of stem cell populations. A common feature of niches is the presence of the extracellular matrix molecule, laminin. Therefore, this study investigated the effect of exogenous laminin on hNSC growth.  * Results: To measure hNSC growth, we established culture conditions using B27-supplemented medium that enable neurospheres to grow from human neural cells plated at clonal densities. Limiting dilution assays confirmed that neurospheres were derived from single cells at these densities. Laminin was found to increase hNSC numbers as measured by this neurosphere formation. The effect of laminin was to augment the proliferation/survival of the hNSC, rather than promoting the undifferentiated state. In agreement, apoptosis was reduced in dissociated neurospheres by laminin in an integrin β1-dependent manner. * Conclusion: The addition of laminin to the culture medium enhances the growth of hNSC, and may therefore aid their large-scale production.

Item Type: Article
Additional Information: Funding Information: This study was supported by the Multiple Sclerosis Society (P.E.H.), the NIH-Cambridge Graduate Partnership Program (J.D.L.), the Royal Society (M.A.C.), and the Wellcome Trust (C. ff.-C.). We are grateful to Dr Nigel Miller (Wellcome Trust) for his help and advice with the flow cytometry and to Dr Pia Siljander (British Heart Foundation) for her help with the platelet adhesion assays.
Uncontrolled Keywords: neuroscience(all),cellular and molecular neuroscience,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2800
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 16 Jul 2022 11:30
Last Modified: 23 Oct 2022 03:57
DOI: 10.1186/1471-2202-9-71

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