α9 integrin promotes neurite outgrowth on tenascin-C and enhances sensory axon regeneration

Andrews, Melissa R., Czvitkovich, Stefan, Dassie, Elisa, Vogelaar, Christina F., Faissner, Andreas, Blits, Bas, Gage, Fred H., Ffrench-Constant, Charles ORCID: https://orcid.org/0000-0002-5621-3377 and Fawcett, James W. (2009) α9 integrin promotes neurite outgrowth on tenascin-C and enhances sensory axon regeneration. Journal of Neuroscience, 29 (17). pp. 5546-5557. ISSN 0270-6474

Full text not available from this repository. (Request a copy)

Abstract

Damaged CNS axons are prevented from regenerating by an environment containing many inhibitory factors. They also lack an integrin that interacts with tenascin-C, the main extracellular matrix glycoprotein of the CNS, which is upregulated after injury. The α9β1 integrin heterodimer is a receptor for the nonalternatively spliced region of tenascin-C, but the α9 subunit is absent in adult neurons. In this study, we show that PC12 cells and adult rat dorsal root ganglion (DRG) neurons do not extend neurites on tenascin-C. However, after forced expression of α9 integrin, extensive neurite outgrowth from PC12 cells and adult rat DRG neurons occurs. Moreover, both DRG neurons and PC12 cells secrete tenascin-C, enabling α9-transfected cells to grow axons on tissue culture plastic. Using adeno-associated viruses to express α9 integrin in vivo in DRGs, we examined axonal regeneration after cervical dorsal rhizotomy or dorsal column crush in the adult rat. After rhizotomy, significantly more dorsal root axons regrew into the dorsal root entry zone at 6 weeks after injury in α9 integrin-expressing animals than in green fluorescent protein (GFP) controls. Similarly, after a dorsal column crush injury, there was significantly more axonal growth into the lesion site compared with GFP controls at 6 weeks after injury. Behavioral analysis after spinal cord injury revealed that both experimental and control groups had an increased withdrawal latency in response to mechanical stimulation when compared with sham controls; however, in response to heat stimulation, normal withdrawal latencies returned after α9 integrin treatment but remained elevated in control groups.

Item Type: Article
Uncontrolled Keywords: neuroscience(all) ,/dk/atira/pure/subjectarea/asjc/2800
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 16 Jul 2022 11:30
Last Modified: 13 Aug 2022 02:21
URI: https://ueaeprints.uea.ac.uk/id/eprint/86306
DOI: 10.1523/JNEUROSCI.0759-09.2009

Actions (login required)

View Item View Item