β1 integrin maintains integrity of the embryonic neocortical stem cell niche

Loulier, Karine, Lathia, Justin D., Marthiens, Veronique, Relucio, Jenne, Mughal, Mohamed R., Tang, Sung Chun, Coksaygan, Turhan, Hall, Peter E., Chigurupati, Srinivasulu, Patton, Bruce, Colognato, Holly, Rao, Mahendra S., Mattson, Mark P., Haydar, Tarik F. and Ffrench-Constant, Charles ORCID: https://orcid.org/0000-0002-5621-3377 (2009) β1 integrin maintains integrity of the embryonic neocortical stem cell niche. PLoS Biology, 7 (8). ISSN 1544-9173

Full text not available from this repository. (Request a copy)

Abstract

During embryogenesis, the neural stem cells (NSC) of the developing cerebral cortex are located in the ventricular zone (VZ) lining the cerebral ventricles. They exhibit apical and basal processes that contact the ventricular surface and the pial basement membrane, respectively. This unique architecture is important for VZ physical integrity and fate determination of NSC daughter cells. In addition, the shorter apical process is critical for interkinetic nuclear migration (INM), which enables VZ cell mitoses at the ventricular surface. Despite their importance, the mechanisms required for NSC adhesion to the ventricle are poorly understood. We have shown previously that one class of candidate adhesion molecules, laminins, are present in the ventricular region and that their integrin receptors are expressed by NSC. However, prior studies only demonstrate a role for their interaction in the attachment of the basal process to the overlying pial basement membrane. Here we use antibody-blocking and genetic experiments to reveal an additional and novel requirement for laminin/integrin interactions in apical process adhesion and NSC regulation. Transient abrogation of integrin binding and signalling using blocking antibodies to specifically target the ventricular region in utero results in abnormal INM and alterations in the orientation of NSC divisions. We found that these defects were also observed in laminin a2 deficient mice. More detailed analyses using a multidisciplinary approach to analyse stem cell behaviour by expression of fluorescent transgenes and multiphoton time-lapse imaging revealed that the transient embryonic disruption of laminin/integrin signalling at the VZ surface resulted in apical process detachment from the ventricular surface, dystrophic radial glia fibers, and substantial layering defects in the postnatal neocortex. Collectively, these data reveal novel roles for the laminin/integrin interaction in anchoring embryonic NSCs to the ventricular surface and maintaining the physical integrity of the neocortical niche, with even transient perturbations resulting in long-lasting cortical defects.

Item Type: Article
Uncontrolled Keywords: neuroscience(all),biochemistry, genetics and molecular biology(all),immunology and microbiology(all),agricultural and biological sciences(all) ,/dk/atira/pure/subjectarea/asjc/2800
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 16 Jul 2022 11:30
Last Modified: 13 Aug 2022 02:21
URI: https://ueaeprints.uea.ac.uk/id/eprint/86302
DOI: 10.1371/journal.pbio.1000176

Actions (login required)

View Item View Item