Individual oligodendrocytes have only a few hours in which to generate new myelin sheaths invivo

Czopka, Tim, ffrench-Constant, Charles ORCID: https://orcid.org/0000-0002-5621-3377 and Lyons, David A. (2013) Individual oligodendrocytes have only a few hours in which to generate new myelin sheaths invivo. Developmental Cell, 25 (6). pp. 599-609. ISSN 1534-5807

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Abstract

The number of myelin sheaths made by individual oligodendrocytes regulates the extent of myelination, which profoundly affects central nervous system function. It remains unknown when, during their life, individual oligodendrocytes can regulate myelin sheath number invivo. We show, using live imaging in zebrafish, that oligodendrocytes make new myelinsheaths during a period of just 5hr, with regulation of sheath number after this time limited to occasional retractions. We also show that activation and reduction of Fyn kinase in oligodendrocytes increases and decreases sheath number per cell, respectively. Interestingly, these oligodendrocytes also generate their new myelin sheaths within the same period, despite having vastly different extents of myelination. Our data demonstrate a restricted time window relative to the lifetime of the individual oligodendrocyte, during which myelin sheath formation occurs and the number of sheaths is determined.

Item Type: Article
Additional Information: Funding Information: We thank Carl Tucker, Paul Wright, and Patricia Smart for fishroom support and Chris Jopling for information about the fyn MO. We would also like to thank Crerar hotels and Sally Womersley for helping to provide microscopes. A very special thanks goes to our late and great colleague Chi-Bin Chien and the Chien laboratory for the tol2kit and to Len Zon for the casper line. We are grateful to Peter Brophy, Ben Emery, Kelly Monk, William Talbot, and members of the Lyons and ffrench-Constant laboratories for critical reading of the manuscript. This work was supported by a David Phillips Fellowship from the BBSRC, a Research Prize from the Lister Institute, and an International Reintegration Grant (to D.A.L.), an EMBO Long-Term Fellowship (to T.C.), and a Wellcome Trust Programme Grant (to C.ff.-C.).
Uncontrolled Keywords: molecular biology,biochemistry, genetics and molecular biology(all),developmental biology,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 16 Jul 2022 00:26
Last Modified: 12 Aug 2022 05:38
URI: https://ueaeprints.uea.ac.uk/id/eprint/86275
DOI: 10.1016/j.devcel.2013.05.013

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