The late response of rat subependymal zone stem and progenitor cells to stroke is restricted to directly affected areas of their niche

Kazanis, Ilias, Gorenkova, Natalia, Zhao, Jing Wei, Franklin, Robin J.M., Modo, Michel and ffrench-Constant, Charles ORCID: https://orcid.org/0000-0002-5621-3377 (2013) The late response of rat subependymal zone stem and progenitor cells to stroke is restricted to directly affected areas of their niche. Experimental Neurology, 248. pp. 387-397. ISSN 0014-4886

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Abstract

Ischaemia leads to increased proliferation of progenitors in the subependymal zone (SEZ) neurogenic niche of the adult brain and to generation and migration of newborn neurons. Here we investigated the spatiotemporal characteristics of the mitotic activity of adult neural stem and progenitor cells in the SEZ during the sub-acute and chronic post-ischaemic phases. Ischaemia was induced by performing a 1. h unilateral middle cerebral artery occlusion (MCAO) and tissue was collected 4/5. weeks and 1. year after the insult. Neural stem cells (NSCs) responded differently from their downstream progenitors to MCAO, with NSCs being activated only transiently whilst progenitors remain activated even at 1. year post-injury. Importantly, mitotic activation was observed only in the affected areas of the niche and specifically in the dorsal half of the SEZ. Analysis of the topography of mitoses, in relation to the anatomy of the lesion and to the position of ependymal cells and blood vessels, suggested an interplay between lesion-derived recruiting signals and the local signals that normally control proliferation in the chronic post-ischaemic phase.

Item Type: Article
Additional Information: Funding Information: This work was funded by an NIH—National Institute of Biomedical Imaging and Bioengineering Quantum Grant Project [ 1P20EB00706 ] to ΜΜ and Cff-C, a BBSRC UK grant ( BB/I013210/1 ) to RF and IK and by a Wellcome Trust, Value in People fellowship to IK.
Uncontrolled Keywords: ischaemia,neural stem cells,neurogenesis,progenitors,proliferation,stroke,subventricular zone,neurology,developmental neuroscience ,/dk/atira/pure/subjectarea/asjc/2800/2808
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 16 Jul 2022 00:26
Last Modified: 16 Jul 2022 00:26
URI: https://ueaeprints.uea.ac.uk/id/eprint/86274
DOI: 10.1016/j.expneurol.2013.06.025

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