Isolation of transposon Tn 5 insertion mutants of Rhodobacter capsulatus unable to reduce trimethylamine-N-oxide and dimethylsulphoxide

Kelly, D. J., Richardson, D. J. ORCID: https://orcid.org/0000-0002-6847-1832, Ferguson, S. J. and Jackson, J. B. (1988) Isolation of transposon Tn 5 insertion mutants of Rhodobacter capsulatus unable to reduce trimethylamine-N-oxide and dimethylsulphoxide. Archives of Microbiology, 150 (2). pp. 138-144. ISSN 0302-8933

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Abstract

1) Rhodobacter capsulatus (formerly Rhodopseudomonas capsulata) strain 37b4 was subjected to transposon Tn 5 mutagenesis. 2) Kanamycin-resistant transconjugants were screened for their inability to reduce trimethylamine-N-oxide (TMAO) as judged by the lack of alkali production during anaerobic growth on plates containing glucose as carbon source and cresol red as pH indicator. 3) Of 6 mutants examined, all were found to have considerably decreased levels of methylviologen-dependent TMAO reductase activity and dimethylsulphoxide (DMSO) reductase activity. 4) Periplasmic fractions of one of these mutants (DK9) and of the parent strain were subjected to sodium dodecylsulphate polyacrylamide gel electrophoresis. The gels were stained for TMAO-reductase and DMSO-reductase. With the wild-type strain, only a single polypeptide band, Mr=46,000, stained for TMAO and DMSO reductase activity. In mutant DK9 this band was not detectable. 5) In contrast to the parent strain, harvested washed cells of mutant DK9 were unable to generate a cytoplasmic membrane potential in the presence of TMAO or DMSO under dark anaerobic conditions. 6) In contrast to the parent strain, DK9 was unable to grow in dark anaerobic culture with fructose as the carbon source and TMAO as oxidant.

Item Type: Article
Uncontrolled Keywords: anaerobic respiration,dmso reduction,electron transport,photosynthetic bacteria (rhodobacter capsulatus),tmao reduction,transposon mutagenesis,microbiology,biochemistry,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:
Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 15 Jul 2022 13:30
Last Modified: 16 Jul 2022 00:25
URI: https://ueaeprints.uea.ac.uk/id/eprint/86212
DOI: 10.1007/BF00425153

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