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Richardson, D. J. 
ORCID: https://orcid.org/0000-0002-6847-1832, Kelly, D. J., Jackson, J. B., Ferguson, S. J. and Alef, K.
(1986)
Inhibitory effects of myxothiazol and 2-n-heptyl-4-hydroxyquinoline-N-oxide on the auxiliary electron transport pathways of Rhodobacter capsulatus.
Archives of Microbiology, 146 (2).
pp. 159-165.
ISSN 0302-8933
Abstract
The effects of various electron transport inhibitors upon the rates of reduction NO3-, dimethyl sulphoxide (DMSO) and N2O in anaerobic suspensions of Rhodobacter capsulatus have been studied. A new method for the determination of the rates of reduction of these auxiliary oxidants in intact cells is presented, based on the proportionality observed between the concentration of oxidant and the duration of the electrochromic carotenoid bandshift. For NO3-and N2O good agreement was found between rates of reduction determined using electrodes and those determined by the electrochromic method. Myxothiazol and antimycin A had no effect on the rates of reduction of NO3-and DMSO suggesting that the cytochrome b/c1complex is not involved in electron transport to these oxidants. 2-n-heptyl-4-hydroxyquinoline-N-oxide (HOQNO) inhibited at two sites, one within the cytochrome b/c1complex and the other on the nitrate reducing pathay, but had no effect on electron transport to N2O or DMSO. In both intact cells and cell free extracts, HOQNO had no effect on the nitrate dependent re-oxidation of reduced methylviologen (MVH2), a direct electron donor to nitrate reductase. Our data are consistent with a branch point for the auxiliary electron transport pathways at the level of the ubiquinone pool.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 2-n-heptyl-4-hydroxyquinoline-n-oxide,auxiliary electron transport,myxothiazol,nitrate reduction,rhodobacter capsulatus,ubiquinone pool,microbiology,biochemistry,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 15 Jul 2022 13:30 |
| Last Modified: | 15 May 2023 00:55 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/86209 |
| DOI: | 10.1007/BF00402344 |
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