Inhibitory effects of myxothiazol and 2-n-heptyl-4-hydroxyquinoline-N-oxide on the auxiliary electron transport pathways of Rhodobacter capsulatus

Richardson, D. J. ORCID: https://orcid.org/0000-0002-6847-1832, Kelly, D. J., Jackson, J. B., Ferguson, S. J. and Alef, K. (1986) Inhibitory effects of myxothiazol and 2-n-heptyl-4-hydroxyquinoline-N-oxide on the auxiliary electron transport pathways of Rhodobacter capsulatus. Archives of Microbiology, 146 (2). pp. 159-165. ISSN 0302-8933

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Abstract

The effects of various electron transport inhibitors upon the rates of reduction NO3-, dimethyl sulphoxide (DMSO) and N2O in anaerobic suspensions of Rhodobacter capsulatus have been studied. A new method for the determination of the rates of reduction of these auxiliary oxidants in intact cells is presented, based on the proportionality observed between the concentration of oxidant and the duration of the electrochromic carotenoid bandshift. For NO3-and N2O good agreement was found between rates of reduction determined using electrodes and those determined by the electrochromic method. Myxothiazol and antimycin A had no effect on the rates of reduction of NO3-and DMSO suggesting that the cytochrome b/c1complex is not involved in electron transport to these oxidants. 2-n-heptyl-4-hydroxyquinoline-N-oxide (HOQNO) inhibited at two sites, one within the cytochrome b/c1complex and the other on the nitrate reducing pathay, but had no effect on electron transport to N2O or DMSO. In both intact cells and cell free extracts, HOQNO had no effect on the nitrate dependent re-oxidation of reduced methylviologen (MVH2), a direct electron donor to nitrate reductase. Our data are consistent with a branch point for the auxiliary electron transport pathways at the level of the ubiquinone pool.

Item Type: Article
Uncontrolled Keywords: 2-n-heptyl-4-hydroxyquinoline-n-oxide,auxiliary electron transport,myxothiazol,nitrate reduction,rhodobacter capsulatus,ubiquinone pool,microbiology,biochemistry,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 15 Jul 2022 13:30
Last Modified: 13 Aug 2022 02:20
URI: https://ueaeprints.uea.ac.uk/id/eprint/86209
DOI: 10.1007/BF00402344

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