Isolation and characterisation of a strain of Pseudomonas putida that can express a periplasmic nitrate reductase

Carter, Jon P., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 and Spiro, Stephen (1995) Isolation and characterisation of a strain of Pseudomonas putida that can express a periplasmic nitrate reductase. Archives of Microbiology, 163 (3). pp. 159-166. ISSN 0302-8933

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Abstract

A strain of Pseudomonas putida that can express a nitrate reductase that is located in the periplasmic compartment was isolated from freshwater. The enzyme was active in vivo during arginine fermentation and at the onset of oxygen limitation in batch cultures. The activity of the enzyme increased the yield of bacteria following fermentative growth under anoxic conditions with arginine, but nitrate reduction did not support growth on nonfermentable carbon substrates under anoxic conditions. Cells expressing the periplasmic nitrate reductase were capable of reducing nitrate in the presence of oxygen. Nitrate reduction under oxic conditions was clearly coupled to a respiratory electron transport chain because: (1) the process was sensitive to the respiratory inhibitors rotenone and 2-n-heptyl-4-hydroxyquinoline N-oxide, and (2) membrane-bound and periplasmic cytochromes were involved. This is the first report of the presence of a periplasmic nitrate reductase in a member of the γ proteobacteria.

Item Type: Article
Uncontrolled Keywords: aerobic nitrate respiration,denitrification,nitrate reductase,periplasm,pseudomonas putida,microbiology,biochemistry,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 15 Jul 2022 12:30
Last Modified: 16 Jul 2022 00:25
URI: https://ueaeprints.uea.ac.uk/id/eprint/86202
DOI: 10.1007/BF00305348

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