Soil and sediment bacteria capable of aerobic nitrate respiration

Carter, J. P., Ya Hsin Hsiao, Hsin Hsiao, Spiro, S. and Richardson, D. J. ORCID: https://orcid.org/0000-0002-6847-1832 (1995) Soil and sediment bacteria capable of aerobic nitrate respiration. Applied and Environmental Microbiology, 61 (8). pp. 2852-2858. ISSN 0099-2240

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Abstract

Several laboratory strains of gram-negative bacteria are known to be able to respire nitrate in the presence of oxygen, although the physiological advantage gained from this process is not entirely clear. The contribution that aerobic nitrate respiration makes to the environmental nitrogen cycle has not been studied. As a first step in addressing this question, a strategy, which allows for the isolation of organisms capable of reducing nitrate to nitrite following aerobic growth has been developed. Twenty-nine such strains have been isolated from three soils and a freshwater sediment and shown to comprise members of three genera (Pseudomonas, Aeromonas, and Moraxella). All of these strains expressed a nitrate reductase with an active site located in the periplasmic compartment. Twenty-two of the strains showed significant rates of nitrate respiration in the presence of oxygen when assayed with physiological electron donors. Also isolated was one member of the gram-positive genus Arthrobacter, which was likewise able to respire nitrate in the presence of oxygen but appeared to express a different type of nitrate reductase. In the four environments studied, culturable bacteria capable of aerobic nitrate respiration were isolated in significant numbers (104 to 107 per g of soil or sediment) and in three cases were as abundant as, or more abundant than, culturable bacteria capable of denitrification. Thus, it seems likely that the corespiration of nitrate and oxygen may indeed make a significant contribution to the flux of nitrate to nitrite in the environment.

Item Type: Article
Uncontrolled Keywords: biotechnology,food science,ecology,applied microbiology and biotechnology ,/dk/atira/pure/subjectarea/asjc/1300/1305
Faculty \ School:
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 15 Jul 2022 12:30
Last Modified: 21 Jul 2022 14:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/86201
DOI: 10.1128/aem.61.8.2852-2858.1995

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