Optical biosensing of nitrate ions using a sol-gel immobilized nitrate reductase

Aylott, Jonathan W., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 and Russell, David A. (1997) Optical biosensing of nitrate ions using a sol-gel immobilized nitrate reductase. Analyst, 122 (1). pp. 77-80. ISSN 0003-2654

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The coupling of enzymes with sol-gel technology creates exciting possibilities-for biosensing. Enzymes can be highly selective and will only respond to specific analytes. Sol-gels provide a unique matrix in which various biomaterials can be immobilized without any loss of enzyme activity. These two components have been combined for the optical biosensing of nitrate ions. The periplasmic nitrate reductase (Nap) extracted from the denitrifying bacterium Thiosphaera pantotropha reacts specifically with the-nitrate (NO3-) anion. The encapsulation of this enzyme in a sol-gel structure for the optical biosensing of nitrate ions is reported. The reduction of nitrate, by periplasmic nitrate reductase results in a characteristic change in the UV/VIS absorption spectrum of the nitrate reductase. This spectroscopic change has been quantitatively calibrated against nitrate concentration. The nitrate biosensing system is fully reversible and is highly sensitive and selective to nitrate-ions. The results obtained show that the activity of the enzyme is not affected by the sol-gel matrix, even after a storage period of up to six months. As no leaching of the Nap from the sol-gel matrix was observed, it is clear that the encapsulation of this nitrate sensitive enzyme in a sol-gel medium represents an ideal anionic recognition element of an optical biosensor for the detection of nitrate ions in the μmol l-1 range.

Item Type: Article
Uncontrolled Keywords: enzyme,nitrate,nitrate reductase,optical biosensing,sol-gel,analytical chemistry,biochemistry,environmental chemistry,spectroscopy,electrochemistry ,/dk/atira/pure/subjectarea/asjc/1600/1602
Faculty \ School:
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Organisms and the Environment
Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 15 Jul 2022 12:30
Last Modified: 14 May 2023 13:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/86198
DOI: 10.1039/a606146j

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