Identification of an assimilatory nitrate reductase in mutants of Paracoccus denitrificans GB17 deficient in nitrate respiration

Sears, Heather J., Little, Phillip J., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832, Berks, Ben C., Spiro, Stephen and Ferguson, Stuart J. (1997) Identification of an assimilatory nitrate reductase in mutants of Paracoccus denitrificans GB17 deficient in nitrate respiration. Archives of Microbiology, 167 (1). pp. 61-66. ISSN 0302-8933

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Abstract

A Paracoccus denitrificans strain (M6Ω) unable to use nitrate as a terminal electron acceptor was constructed by insertional inactivation of the periplasmic and membrane-bound nitrate reductases. The mutant strain was able to grow aerobically with nitrate as the sole nitrogen source. It also grew anaerobically with nitrate as sole nitrogen source when nitrous oxide was provided as a respiratory electron acceptor. These growth characteristics are attributed to the presence of a third, assimilatory nitrate reductase. Nitrate reductase activity was detectable in intact cells and soluble fractions using nonphysiological electron donors. The enzyme activity was not detectable when ammonium was included in the growth medium. The results provide an unequivocal demonstration that P. denitrificans can express an assimilatory nitrate reductase in addition to the well-characterised periplasmic and membrane-bound nitrate reductases.

Item Type: Article
Additional Information: Funding Information: Acknowledgement This research was supported by grant 83/ CO2012 from the Biotechnology and Biological Sciences Research Council (BBSRC) to D. J. Richardson and S. J. Ferguson.
Uncontrolled Keywords: nitrate assimilation,nitrate reductase,paracoccus denitrificans,thiosphaera pantotropha,microbiology,biochemistry,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:
Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 15 Jul 2022 12:30
Last Modified: 16 Jul 2022 00:25
URI: https://ueaeprints.uea.ac.uk/id/eprint/86197
DOI: 10.1007/s002030050417

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