Detection of genes for membrane-bound nitrate reductase in nitrate-respiring bacteria and in community DNA

Gregory, Lisa G., Karakas-Sen, Asuman, Richardson, David J. ORCID: and Spiro, Stephen (2000) Detection of genes for membrane-bound nitrate reductase in nitrate-respiring bacteria and in community DNA. FEMS Microbiology Letters, 183 (2). pp. 275-279. ISSN 0378-1097

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A nested PCR primed by four degenerate oligonucleotides was developed for the specific amplification of sequences from the narG gene encoding the membrane-bound nitrate reductase. This approach was used to amplify fragments of the narG gene from five Pseudomonas species previously shown to be able to express the membrane-bound nitrate reductase and from community DNA extracted from a freshwater sediment. Amino acid sequences encoded by the narG fragments were compared to one another, and to the corresponding regions of related enzymes. This comparison indicates that the amplification protocols are specific for their intended targets. Sequences amplified from community DNA were tightly clustered, which may indicate a degree of homogeneity in the sediment community. The PCR primers and amplification protocols described will be useful in future studies of nitrate respiring populations.

Item Type: Article
Additional Information: Funding Information: This work was supported by BBSRC and Royal Society grants to D.J.R. and S.S., a NERC studentship to L.G.G. and a Turkish Government grant to A.K.S. We are grateful to N. Shearer and Dr B. Berks for helpful discussions.
Uncontrolled Keywords: denitrification,membrane-bound nitrate reductase,nitrate respiration,microbiology,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Organisms and the Environment
Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
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Depositing User: LivePure Connector
Date Deposited: 14 Jul 2022 16:31
Last Modified: 15 May 2023 00:54
DOI: 10.1016/S0378-1097(99)00667-9

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