Introduction to the Biochemistry and Molecular Biology of Denitrification

van Spanning, Rob J.M., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 and Ferguson, Stuart J. (2007) Introduction to the Biochemistry and Molecular Biology of Denitrification. In: Biology of the Nitrogen Cycle. Elsevier, pp. 3-20. ISBN 9780444528575

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Abstract

This chapter provides an overview of the biochemistry and genetics of denitrification in such organisms. It considers the aspects of denitrification that occur in archaea and certain fungi. Denitrification has been mostly studied in Paracoccus denitrificans and Pseudomonas stutzeri and so it describes denitrification for each of these organisms in turn before considering to what extent general principles can be discerned. In recent years, high-resolution crystal structures have become available for these enzymes with the exception of the structure for NO-reductase. In general, the proteins required for denitrification are only produced under (close to) anaerobic conditions, and if anaerobically grown, cells are exposed to O2 and then the activities of the proteins are inhibited. Specialized denitrifiers, such as P. denitrificans and the denitrifying Pseudomonads, contain more than 40 genes, which encode the proteins that make up a full denitrification pathway. They include the structural genes for the enzymes and e- donors, their regulators as well as many accessory genes required for assembly, cofactor synthesis, and insertion into the enzymes. In contrast, some denitrifiers can only carry out the two central reactions of the pathway and use these activities to support growth, but the cost of maintaining this capability is a very small amount of genome space. It provides insights into the regulation of gene expression and the way in which some denitrification enzymes play different roles in bacteria.

Item Type: Book Section
Uncontrolled Keywords: agricultural and biological sciences(all) ,/dk/atira/pure/subjectarea/asjc/1100
Faculty \ School:
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 14 Jul 2022 16:30
Last Modified: 14 Jul 2022 16:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/86113
DOI: 10.1016/B978-044452857-5.50002-3

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