Insights into methionine S-methylation in diverse organisms

Peng, Ming, Li, Chun-Yang, Chen, Xiu-Lan, Williams, Beth ORCID:, Kang, Li, Gao, Ya-Nan, Wang, Peng, Wang, Ning, Gao, Chao, Zhang, Shan, Schoelmerich, Marie C., Banfield, Jillian F., Miller, J. Benjamin ORCID:, Le Brun, Nick E. ORCID:, Todd, Jonathan D. and Zhang, Yu-Zhong (2022) Insights into methionine S-methylation in diverse organisms. Nature Communications, 13. ISSN 2041-1723

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Dimethylsulfoniopropionate (DMSP) is an important marine anti-stress compound, with key roles in global nutrient cycling, chemotaxis and, potentially, climate regulation. Recently, diverse marine Actinobacteria, α- and γ-proteobacteria were shown to initiate DMSP synthesis via the methionine (Met) S-methyltransferase enzyme (MmtN), generating S-methyl-Met (SMM). Here we characterize a roseobacterial MmtN, providing structural and mechanistic insights into this DMSP synthesis enzyme. We propose that MmtN uses the proximity and desolvation mechanism for Met S-methylation with two adjacent MmtN monomers comprising the Met binding site. We also identify diverse functional MmtN enzymes in potentially symbiotic archaeal Candidatus Woesearchaeota and Candidate Phyla Radiation (CPR) bacteria, and the animalcule Adineta steineri, not anticipated to produce SMM and/or DMSP. These diverse MmtN enzymes, alongside the larger plant MMT enzyme with an N-terminus homologous to MmtN, likely utilize the same proximity and desolvation mechanism. This study provides important insights into the catalytic mechanism of SMM and/or DMSP production, and proposes roles for these compounds in secondary metabolite production, and SMM cycling in diverse organisms and environments.

Item Type: Article
Additional Information: Acknowledgements: The authors thank the staff from BL17U1, BL18U1&BL19U1 beamlines of the National Facility for Protein Sciences Shanghai (NFPS) and Shanghai Synchrotron Radiation Facility, for assistance during data collection. They also thank Caiyun Sun, Chengjia Zhang, Guannan Lin, Xiaoju Li, Jingyao Qu, Xiangmei Ren from the State Key Laboratory of Microbial Technology of Shandong University for help and guidance with the single crystal X-ray diffractometer, HPLC, LC–MS, and ion chromatography. This work was supported by: the National Science Foundation of China (grants 91851205 awarded to Y.-Z.Z., 42076229 awarded to C.-Y.L., 31800107 awarded to P.W., 31961133016 awarded to Y.-Z.Z., and 42106142 awarded to M.P.), the National Key Research and Development Program of China (2018YFC1406700 awarded to Y.-Z.Z., 2021YFA0909600 awarded to C.-Y.L.), the Fundamental Research Funds for the Central Universities (202172002 awarded to Y.-Z.Z., 202041011 awarded to C.-Y.L.), the Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (2019JZZY010817 awarded to Y.-Z.Z.), the Program of Shandong for Taishan Scholars (tspd20181203 awarded to Y.-Z.Z.), AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology (2017ASTCP-OS14 awarded to Y.-Z.Z.), China Postdoctoral Science Foundation (2021M691914 awarded to M.P.), Natural Science Foundation of Shandong Province (ZR2021QD071 awarded to M.P.), the United Kingdom’s Natural and Environmental Research Council (NERC, NE/P012671/1 awarded to J.D.T., NE/N002385/1 awarded to J.D.T., and NE/V000756/1 awarded to J.B.M. and J.D.T.), and the United Kingdom’s Biotechnology and Biological Sciences Research Council (BBSRC, BB/P006140/1 awarded to N.E.L.).
Uncontrolled Keywords: physics and astronomy(all),chemistry(all),biochemistry, genetics and molecular biology(all),sdg 14 - life below water,sdg 13 - climate action ,/dk/atira/pure/subjectarea/asjc/3100
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Science > Research Groups > Plant Sciences
Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Groups > Chemistry of Life Processes
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
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Depositing User: LivePure Connector
Date Deposited: 01 Jun 2022 11:30
Last Modified: 22 Oct 2022 18:32
DOI: 10.1038/s41467-022-30491-5

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