Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1

Fan, Li, Fröhlich, Katja, Melzer, Eric, Pruitt, Rory N., Albert, Isabell, Zhang, Lisha, Joe, Anna, Hua, Chenlei, Song, Yanyue, Albert, Markus, Kim, Sang Tae, Weigel, Detlef, Zipfel, Cyril, Chae, Eunyoung, Gust, Andrea A. and Nürnberger, Thorsten (2022) Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1. Nature Communications, 13. ISSN 2041-1723

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Abstract

Activation of plant pattern-triggered immunity (PTI) relies on the recognition of microbe-derived structures, termed patterns, through plant-encoded surface-resident pattern recognition receptors (PRRs). We show that proteobacterial translation initiation factor 1 (IF1) triggers PTI in Arabidopsis thaliana and related Brassicaceae species. Unlike for most other immunogenic patterns, IF1 elicitor activity cannot be assigned to a small peptide epitope, suggesting that tertiary fold features are required for IF1 receptor activation. We have deployed natural variation in IF1 sensitivity to identify Arabidopsis leucine-rich repeat (LRR) receptor-like protein 32 (RLP32) as IF1 receptor using a restriction site-associated DNA sequencing approach. RLP32 confers IF1 sensitivity to rlp32 mutants, IF1-insensitive Arabidopsis accessions and IF1-insensitive Nicotiana benthamiana, binds IF1 specifically and forms complexes with LRR receptor kinases SOBIR1 and BAK1 to mediate signaling. Similar to other PRRs, RLP32 confers resistance to Pseudomonas syringae, highlighting an unexpectedly complex array of bacterial pattern sensors within a single plant species.

Item Type: Article
Additional Information: Acknowledgements: Research in the lab of T.N. was funded by DFG grants Nu70/9-1, Nu70/9-2, Nu 70/15-1, Nu70/16-1, Nu70/17-1, and SFB1101. RAD-seq work was supported by the Max Planck Society (to D.W.) and the Academic Research Fund (MOE2019-T2-1-134) from the Ministry of Education, Singapore (to E.C.). We thank Caterina Brancato for assistance in plant transformation, and Mirita Franz-Wachtel and Ilya Bezrukov for processing proteomics and RAD-Seq data. Open Access funding enabled and organized by Projekt DEAL.
Uncontrolled Keywords: chemistry(all),biochemistry, genetics and molecular biology(all),physics and astronomy(all) ,/dk/atira/pure/subjectarea/asjc/1600
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Depositing User: LivePure Connector
Date Deposited: 09 May 2022 09:30
Last Modified: 09 May 2022 09:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/84965
DOI: 10.1038/s41467-022-28887-4

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