Genome characterisation of an isoprene-degrading Alcaligenes sp. isolated from a tropical restored forest

Uttarotai, Toungporn, Sutheeworapong, Sawannee, Crombie, Andrew T., Murrell, J. Colin, Mhuantong, Wuttichai, Noirungsee, Nuttapol, Wangkarn, Sunanta, Bovonsombut, Sakunnee, McGenity, Terry J. and Chitov, Thararat (2022) Genome characterisation of an isoprene-degrading Alcaligenes sp. isolated from a tropical restored forest. Biology, 11 (4). ISSN 2079-7737

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Abstract

Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.

Item Type: Article
Additional Information: Funding Information: This research was funded by Science Achievement Scholarship of Thailand (granted to T.U), Biodiversity-based Economy Development Office (Public Organisation) (BEDO) grant to T.C. and S.B (36/2562), Natural Environment Research Council (NERC) grant to T.J.M. (NE/J009555/1)), European Research Council (ERC) Advanced Grant (IsoMet 694578) to J.C.M. and partially supported by Chiang Mai University, Thailand and the University of Essex, UK.
Uncontrolled Keywords: alcaligenes,climate-active gas,genome,isoprene,isoprene degradation,agricultural and biological sciences(all),biochemistry, genetics and molecular biology(all),immunology and microbiology(all),sdg 15 - life on land,sdg 13 - climate action ,/dk/atira/pure/subjectarea/asjc/1100
Faculty \ School: Faculty of Science > School of Environmental Sciences
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Depositing User: LivePure Connector
Date Deposited: 06 May 2022 03:58
Last Modified: 07 Jun 2022 00:25
URI: https://ueaeprints.uea.ac.uk/id/eprint/84897
DOI: 10.3390/biology11040519

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