Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Yu, Guotai, Matny, Oadi, Champouret, Nicolas, Steuernagel, Burkhard, Moscou, Matthew J., Hernández-Pinzón, Inmaculada, Green, Phon, Hayta, Sadiye, Smedley, Mark, Harwood, Wendy, Kangara, Ngonidzashe, Yue, Yajuan, Gardener, Catherine, Banfield, Mark J., Olivera, Pablo D., Welchin, Cole, Simmons, Jamie, Millet, Eitan, Minz-Dub, Anna, Ronen, Moshe, Avni, Raz, Sharon, Amir, Patpour, Mehran, Justesen, Annemarie F., Jayakodi, Murukarthick, Himmelbach, Axel, Stein, Nils, Wu, Shuangye, Poland, Jesse, Ens, Jennifer, Pozniak, Curtis, Karafiátová, Miroslava, Molnár, István, Doležel, Jaroslav, Ward, Eric R., Reuber, T. Lynne, Jones, Jonathan D. G., Mascher, Martin, Steffenson, Brian J. and Wulff, Brande B. H. (2022) Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62. Nature Communications, 13 (1). ISSN 2041-1723

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Abstract

The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.

Item Type: Article
Additional Information: Funding Information: This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by grants from the 2Blades Foundation, USA, to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme to B.B.H.W. (BBS/E/J/000PR9780); the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; Human Frontier Science Program long-term fellowship (LT000218/2011-L) to M.J.M.; the Gordon and Betty Moore Foundation through grant GBMF4725 to the 2Blades Foundation; and the Gatsby Charitable Foundation to J.D.G.J.
Uncontrolled Keywords: chemistry(all),biochemistry, genetics and molecular biology(all),physics and astronomy(all) ,/dk/atira/pure/subjectarea/asjc/1600
Faculty \ School:
Faculty of Science > School of Biological Sciences
Faculty of Science
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Depositing User: LivePure Connector
Date Deposited: 31 Mar 2022 08:38
Last Modified: 15 Apr 2022 00:52
URI: https://ueaeprints.uea.ac.uk/id/eprint/84369
DOI: 10.1038/s41467-022-29132-8

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