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Christodoulou, Marilena 
ORCID: https://orcid.org/0000-0002-5637-3768, Aspray, Terence J., Piec, Isabelle ORCID: https://orcid.org/0000-0002-0648-1330, Washbourne, Christopher, Tang, Jonathan C. Y. ORCID: https://orcid.org/0000-0001-6305-6333, Fraser, William D. and Schoenmakers, Inez
and
the VDOP Trial group
(2022)
Vitamin D supplementation for 12 months in older adults alters regulators of bone metabolism but does not change Wnt signalling pathway markers.
JBMR Plus, 6 (5).
ISSN 2473-4039
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Abstract
Vitamin D status and supplementation regulates bone metabolism and may modulate Wnt signaling. We studied the response of hormonal regulators of bone metabolism, markers of Wnt signaling and bone turnover and bone mineral density (BMD) and bone mineral content (BMC) in a randomized vitamin D intervention trial (12,000 IU, 24,000 IU, 48,000 IU/mo for 1 year; men and women aged >70 years; n = 379; ISRCTN35648481). Associations with total and free 25(OH)D concentrations were analyzed by linear regression. Baseline vitamin D status was (mean ± SD) 25(OH)D: 40.0 ± 20.1 nmol/L. Supplementation dose-dependently increased total and free 25(OH)D concentrations and decreased plasma phosphate and parathyroid hormone (PTH) (all p < 0.05). The procollagen 1 intact N-terminal (PINP)/C-terminal telopeptide (CTX) ratio, C-terminal fibroblast growth factor-23 (cFGF23), and intact FGF23 (iFGF23) significantly increased with no between-group differences, whereas Klotho was unchanged. 1,25(OH) 2D and PINP significantly increased in the 24 IU and 48,000 IU groups. Sclerostin (SOST), osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL), BMD, BMC, and CTX remained unchanged. Subgroup analyses with baseline 25(OH)D <25 nmol/L (n = 94) provided similar results. Baseline total and free 25(OH)D concentrations were positively associated with 1,25(OH) 2D, 24,25(OH) 2D (p < 0.001), vitamin D binding protein (DBP) (p < 0.05), BMD, and BMC (p < 0.05). Associations with PTH (p <0.001), cFGF23 (p < 0.01), and BAP (p < 0.05) were negative. After supplementation, total and free 25(OH)D concentrations remained positively associated only with 24,25(OH) 2D (p < 0.001) and DBP (p < 0.001) and negatively with estimated glomerular filtration rate (eGFR) (p < 0.01). PTH and SOST were significantly associated only with free 25(OH)D. There were no significant relationships with BMD and BMC after supplementation. The decrease in PTH and increase in PINP/CTX ratio suggest a protective effect of supplementation on bone metabolism, although no significant effect on BMD or pronounced changes in regulators of Wnt signaling were found. The increase in FGF23 warrants caution because of its negative association with skeletal and cardiovascular health. Associations of total and free 25(OH)D with biomarkers were similar and known positive associations between vitamin D status and BMD were confirmed. The change in associations after supplementation might suggest a threshold effect.
| Item Type: | Article |
|---|---|
| Additional Information: | Research Funding: Academy of Medical Sciences. Grant Number: SBF002\1097; Arthritis Research UK. Grant Number: 19544; Medical Research Council. Grant Number: U105960371; University of East Anglia |
| Uncontrolled Keywords: | bone turnover,fibroblast growth factor-23,parathyroid hormone,vitamin d,wnt pathway,endocrinology, diabetes and metabolism,orthopedics and sports medicine ,/dk/atira/pure/subjectarea/asjc/2700/2712 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 08 Mar 2022 16:30 |
| Last Modified: | 19 Oct 2023 03:17 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/83934 |
| DOI: | 10.1002/jbm4.10619 |
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