Enterococcus innesii sp. nov., isolated from the wax moth Galleria mellonella

Gooch, Harriet C. C., Kiu, Raymond, Rudder, Steven, Baker, David J., Hall, Lindsay J. and Maxwell, Anthony (2021) Enterococcus innesii sp. nov., isolated from the wax moth Galleria mellonella. International Journal of Systematic and Evolutionary Microbiology, 71 (12). ISSN 1466-5026

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Abstract

Four bacterial strains were isolated from two different colony sources of the wax moth Galleria mellonella. They were characterized by a polyphasic approach including 16S rRNA gene sequence analysis, core-genome analysis, average nucleotide identity (ANI) analysis, digital DNA–DNA hybridization (dDDH), determination of G+C content, screening of antibiotic resistance genes, and various phenotypic analyses. Initial analysis of 16S rRNA gene sequence identities indicated that strain GAL7T was potentially very closely related to Enterococcus casseliflavus and Enterococcus gallinarum, having 99.5–99.9 % sequence similarity. However, further analysis of whole genome sequences revealed a genome size of 3.69 Mb, DNA G+C content of 42.35 mol%, and low dDDH and ANI values between the genomes of strain GAL7T and closest phylogenetic relative E. casseliflavus NBRC 100478T of 59.0 and 94.5 %, respectively, indicating identification of a putative new Enterococcus species. In addition, all novel strains encoded the atypical vancomycin-resistance gene vanC-4. Results of phylogenomic, physiological and phenotypic characterization confirmed that strain GAL7T represented a novel species within the genus Enterococcus, for which the name Enterococcus innesii sp. nov. is proposed. The type strain is GAL7T (=DSM 112306T=NCTC 14608T).

Item Type: Article
Additional Information: This research was supported in part by the Norwich Bioscience Institutes (NBI) Computing infrastructure for Science (CiS) group through the provision of a High-Performance Computing (HPC) Cluster. Funding Information: H.C.C.G. is funded by the NC3Rs Postgraduate Studentship Scheme (NC/R001782/1). A.M. is supported by Biotechnology and Biosciences Research Council (UK) Institute Strategic Programme Grant BB/ P012523/1, and a Wellcome Trust Investigator Award (110072/Z/15/Z). L.J.H. is supported by Wellcome Trust Investigator Awards 100974 /C/13/Z and 220876/Z/20/Z; the Biotechnology and Biological Sciences Research Council (BBSRC), Institute Strategic Programme Gut Microbes and Health BB/R012490/1, and its constituent projects BBS/E/F/000PR10353 and BBS/E/F/000PR10356.
Uncontrolled Keywords: antibiotic resistance,enterococcus,galleria mellonella,novel species,vancomycin resistant,wax moth,microbiology,ecology, evolution, behavior and systematics ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 17 Jan 2022 11:30
Last Modified: 25 Apr 2022 00:20
URI: https://ueaeprints.uea.ac.uk/id/eprint/83042
DOI: 10.1099/ijsem.0.005168

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