Matrix metalloproteinase distribution during early corneal wound healing

Mulholland, B., Tuft, S. J. and Khaw, P. T. (2005) Matrix metalloproteinase distribution during early corneal wound healing. Eye, 19 (5). pp. 584-588. ISSN 0950-222X

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Aim: To compare matrix metalloproteinase (MMP) localisation in anterior keratectomy (AK) and lamellar keratectomy (LK) wounds. Methods: Wounds were produced in one eye of 24 rabbits. The AK wounds were made to approximately 120 mm in depth and then allowed to re-epithelialise. The LK wounds were of similar depth, but the anterior stroma and epithelium were replaced after a second deeper keratectomy had been performed. Immunohistochemistry was used to localise the MMP-1, -2, -3, and -9 at intervals from 4 h to 14 days following surgery. The contralateral eyes acted as controls. Results: After an AK wound MMP-1 was present at the leading edge of migrating epithelium after 18 h, while MMP-2 and -9 were localised behind the advancing epithelial edge. The presence of these enzymes rapidly fell to low levels after epithelial closure. There was only faint MMP-3 localisation between days 3 and 7. After an LK wound, MMP-1, -3, and -9 were not detected in the stromal interface, but MMP-2 was present at all time points. Conclusions: This study suggests that after an AK wound, MMP-1 is a key mediator of epithelial migration, while MMP-2 and -9, and to a lesser extent MMP-3, may participate in the remodelling of corneal stroma and the reformation of epithelial basement membrane. In contrast, an LK wound results in a much lower stimulus for MMP activation. The action of MMP-2 in stromal repair is thus partly independent of epithelial injury.

Item Type: Article
Uncontrolled Keywords: cornea,corneal epithelium,metalloproteinases,wound healing,in-situ keratomileusis,photorefractive keratectomy,gelatinase-b,gene-expression,iv collagenase,laser,immunolocalization,timp-1,repair,chondrocytes
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: LivePure Connector
Date Deposited: 10 Jan 2022 11:30
Last Modified: 23 Oct 2022 03:27
DOI: 10.1038/sj.eye.6701557

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