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Ahmed, Maqsood, Owens, Matthew J. S., Toledo, Enrique M., Arenas, Ernest, Bradley, Mark and ffrench-Constant, Charles 
ORCID: https://orcid.org/0000-0002-5621-3377
(2021)
Combinatorial ECM arrays identify cooperative roles for matricellular proteins in enhancing the generation of TH+ neurons from human pluripotent cells.
Frontiers in Cell and Developmental Biology, 9.
ISSN 2296-634X
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Abstract
The development of efficient cell culture strategies for the generation of dopaminergic neurons is an important goal for transplantation-based approaches to treat Parkinson’s disease. To identify extracellular matrix molecules that enhance differentiation and might be used in these cell cultures we have used micro-contact printed arrays on glass slides presenting 190 combinations of 19 extracellular matrix molecules selected on the basis of their expression during embryonic development of the ventral midbrain. Using long-term neuroepithelial stem cells (Lt-NES), this approach identified a number of matricellular proteins that enhanced differentiation, with the combination of Sparc, Sparc-like (Sparc-l1) and Nell2 increasing the number of tyrosine hydroxylase+ neurons derived from Lt-NES cells and, critically for further translation, human pluripotent stem cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Dec 2021 16:30 |
| Last Modified: | 23 Oct 2022 03:26 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/82820 |
| DOI: | 10.3389/fcell.2021.755406 |
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