Lipid profiles from dried blood spots reveal lipidomic signatures of newborns undergoing mild therapeutic hypothermia after hypoxic-ischemic encephalopathy

Nixon, Rebekah, Ip, Ting Hin Richard, Jenkins, Benjamin, Yip, Ping K., Clarke, Paul, Ponnusamy, Vennila, Michael-Titus, Adina T., Koulman, Albert and Shah, Divyen K. (2021) Lipid profiles from dried blood spots reveal lipidomic signatures of newborns undergoing mild therapeutic hypothermia after hypoxic-ischemic encephalopathy. Nutrients, 13 (12). ISSN 2072-6643

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Abstract

Hypoxic-ischemic encephalopathy (HIE) is associated with perinatal brain injury, which may lead to disability or death. As the brain is a lipid-rich organ, various lipid species can be significantly impacted by HIE and these correlate with specific changes to the lipidomic profile in the circulation. Objective: To investigate the peripheral blood lipidomic signature in dried blood spots (DBS) from newborns with HIE. Using univariate analysis, multivariate analysis and sPLS-DA modelling, we show that newborns with moderate–severe HIE (n = 46) who underwent therapeutic hypothermia (TH) displayed a robust peripheral blood lipidomic signature comprising 29 lipid species in four lipid classes; namely phosphatidylcholine (PC), lysophosphatidylcholine (LPC), triglyceride (TG) and sphingomyelin (SM) when compared with newborns with mild HIE (n = 18). In sPLS-DA modelling, the three most discriminant lipid species were TG 50:3, TG 54:5, and PC 36:5. We report a reduction in plasma TG and SM and an increase in plasma PC and LPC species during the course of TH in newborns with moderate–severe HIE, compared to a single specimen from newborns with mild HIE. These findings may guide the research in nutrition-based intervention strategies after HIE in synergy with TH to enhance neuroprotection.

Item Type: Article
Additional Information: Funding Information: We are grateful to the Barts Charity, London, UK. We are also grateful to The Neonatal Society, UK for a Student Research bursary for RN, NIHR Cambridge Biomedical Research Centre (146281) & Biotechnology and Biological Sciences Research Council (BB/P028195/1). The funders had no other role in any aspect of this study or its publication.
Uncontrolled Keywords: brain,dried blood spot,hypoxic-ischemic encephalopathy,lipidome,lipids,newborn,nutrition,therapeutic hypothermia,food science,nutrition and dietetics ,/dk/atira/pure/subjectarea/asjc/1100/1106
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 09 Dec 2021 16:30
Last Modified: 05 Jan 2022 13:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/82623
DOI: 10.3390/nu13124301

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