Genomic analysis of a rare recurrent Listeria monocytogenes prosthetic joint infection indicates a protected niche within biofilm on prosthetic materials

Hutchins, Chloe, Sayavedra, Lizbeth, Diaz, Maria, Gupta, Puja, Tissingh, Elizabeth, Elumogo, Chiamaka, Nolan, John, Charles, Ian, Elumogo, Ngozi and Narbad, Arjan (2021) Genomic analysis of a rare recurrent Listeria monocytogenes prosthetic joint infection indicates a protected niche within biofilm on prosthetic materials. Scientific Reports, 11 (1). ISSN 2045-2322

[img] PDF (Published_Version) - Published Version
Available under License Creative Commons Attribution.

Download (2MB)

Abstract

Listeria monocytogenes is a rare cause of prosthetic joint infections (PJI). In this study, we describe a case of recurrent L. monocytogenes infections, 39 months apart, following debridement and retention of a prosthetic hip. Despite numerous studies reporting persistent L. monocytogenes in human infections, the genomic and phenotypic changes that clinically relevant strains undergo in the host are poorly understood. Improved knowledge of how PJI occurs is needed to improve the management of prosthetic infections. We used a combination of long- and short-read sequencing to identify any potential genomic differences between two L. monocytogenes isolates that occurred over 39-month incubation in the host. The isolates, QI0054 and QI0055, showed three single nucleotide polymorphisms and three insertions or deletions, suggesting that the recurrent infection was caused by the same strain. To identify potential differences in the capacity for persistence of these isolates, their biofilm-forming ability and potential to colonize prosthesis-relevant materials was investigated both in microtitre plates and on prosthetic material titanium, stainless steel 316 and ultra-high molecular weight polyethylene. Whilst the L. monocytogenes isolate from the most recent infection (QI0055) was able to form higher biofilm in microtitre plates, this did not lead to an increase in biomass on prosthetic joint materials compared to the initial isolate (QI0054). Both clinical isolates were able to form significantly more biofilm on the two metal prosthetic materials than on the ultra-high molecular weight polyethylene, in contrast to reference strain Scott A. Transcriptomics revealed 41 genes overexpressed in biofilm state and 643 in planktonic state. Moreover, genes with mutations were actively expressed in both isolates. We conclude the isolates are derived from the same strain and hypothesize that L. monocytogenes formed biofilm on the prosthetic joint materials, with minimal exposure to stresses, which permitted their survival and growth.

Item Type: Article
Additional Information: Acknowledgements: We thank David Baker for library preparation and whole-genome sequencing. We thank Rekha Panwar for contributions towards culturing the clinical isolates and Antonietta Hayhoe for her assistance in ensuring study adherence with ethical guidelines. This work was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) Microbes in the Food Chain BBS/E/F/000PR10348 (Theme 1, Epidemiology and Evolution of Pathogens in the Food Chain), Newton Fund Joint Centre Award, and BBSRC Institute Strategic Programme Gut Microbes and Health BB/R012490/1 (BBS/E/F/000PR10355 and BBS/E/F/000PR10356).
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 18 Nov 2021 01:57
Last Modified: 24 Nov 2021 03:14
URI: https://ueaeprints.uea.ac.uk/id/eprint/82188
DOI: 10.1038/s41598-021-01376-2

Actions (login required)

View Item View Item