Functional characterization of MtCBP60B in plant endosymbioses

Bridge, Edmund (2020) Functional characterization of MtCBP60B in plant endosymbioses. Masters thesis, University of East Anglia.

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Plants perceive a range of microbes at the root-soil interface, which induces cellular responses in a microbe-specific way. Legume plants, such as Medicago truncatula, respond to signals from symbiotic nitrogen-fixing rhizobial bacteria or arbuscular mycorrhizal fungi with the induction of nuclear calcium oscillation. This nuclear calcium release is required for activation of downstream genes involved in the symbiont colonisation process and organogenesis of nodule or arbuscule structures. Generation of these calcium oscillations is controlled by a suite of nuclear-localised ion channels, including the cation channel DMI1. In this study, we begin to describe the activity and function of the DMI1 interacting partner, CALMODULIN BINDING PROTEIN60 B (CBP60B). Here, it is suggested that CBP60B is involved in the colonisation of root tissues by symbiont partners. It was found that the formation of symbiosis specific organs was reduced in the mutant cbp60b-1. In symbiont inoculated tissues, CBP60B was shown to be highly expressed at the nodule apex, in cells infected by rhizobia, as well as in AM containing cortical tissues. In non-colonised tissues, expression was limited to the root apical meristem and the vascular-associated pericycle cells, sites involved in rapid cell division, potentially implicating CBP60B in root organogenesis. Additionally, cbp60b-1 mutants were less susceptible to colonisation by the fungal pathogen Fusarium graminearum compared with the wild-type. Related proteins AtCBP60a, AtCBP60g and AtSARD1 are known to be involved in immune signalling. These results demonstrate that CBP60B may function in the relative modulation of symbiosis and immune signalling in response to microbe interaction.

Item Type: Thesis (Masters)
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Chris White
Date Deposited: 20 Oct 2021 10:44
Last Modified: 29 Sep 2023 01:38


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