Biological iron-sulfur clusters: mechanistic insights from mass spectrometry

Crack, Jason and Le Brun, Nick ORCID: (2021) Biological iron-sulfur clusters: mechanistic insights from mass spectrometry. Coordination Chemistry Reviews, 448. ISSN 0010-8545

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Iron-sulfur (FeS) clusters are protein cofactors that are ubiquitous in life, performing a wide range of functions, such as in electron transfer, catalysis and gene regulation. This functional diversity is underpinned by the inherent reactivity of FeS clusters towards redox change and reaction with small molecules such as molecular oxygen and nitric oxide. Such reactivity also presents significant challenges for their study, and difficulties in obtaining sufficient quantities of stable, homogenous FeS protein samples have severely hampered progress in understanding these fascinating proteins. Recently, the application of mass spectrometry, under conditions where the cluster cofactor remains protein-associated, has yielded major new insight, particularly into FeS cluster assembly and the chemistry occurring at the FeS clusters of transcriptional regulators that coordinate the cell’s response to changing conditions, such as availability of O2 and cellular iron status, or the onset of oxidative or nitrosative stress. Here we review this recent progress, highlighting the power of native mass spectrometric approaches for studies of protein cofactors.

Item Type: Article
Uncontrolled Keywords: dna regulation,iron sensing,iron-sulfur cluster,iron-sulfur cluster biogenesis,mass spectrometry,nitric oxide sensing,oxygen sensing,physical and theoretical chemistry,inorganic chemistry,materials chemistry ,/dk/atira/pure/subjectarea/asjc/1600/1606
Faculty \ School: Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Faculty of Science > Research Groups > Chemistry of Life Processes
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Depositing User: LivePure Connector
Date Deposited: 21 Sep 2021 01:35
Last Modified: 23 Oct 2022 03:01
DOI: 10.1016/j.ccr.2021.214171


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