Investigating Ghanaian Allium species for anti-infective and resistance-reversal natural product leads to mitigate multidrug-resistance in tuberculosis

Danquah, Cynthia Amaning, Tetteh, Michael, Amponsah, Isaac Kingsley, Mensah, Abraham Yeboah, Buabeng, Kwame Ohene, Gibbons, Simon and Bhakta, Sanjib (2021) Investigating Ghanaian Allium species for anti-infective and resistance-reversal natural product leads to mitigate multidrug-resistance in tuberculosis. Antibiotics, 10 (8). ISSN 2079-6382

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Abstract

The bulbs of Allium species are a known source of antibacterial phytochemicals. Anti-infective, efflux pump and biofilm inhibitory activities of bulb extracts of selected Ghanaian shallots Allium cepa var aggregatum were evaluated using the HT-SPOTi assay and other whole-cell phenotypic screening techniques to determine their possible mechanisms of action. Ethanol and aqueous extracts of white A. cepa inhibited the growth of Mycobacterium smegmatis mc2 155 and Escherichia coli, respectively. The majority of the Allium extracts significantly (p < 0.05) exhibited efflux pump inhibitory activity against all the acid-fast, Gram-positive and Gram-negative strains used. Hexane and chloroform extract of the pink A. cepa and the aqueous extract of the white A. cepa significantly inhibited M. smegmatis biofilm formation. For Pseudomonas aeruginosa, the inhibition was observed at 250 µg/mL for the aqueous extract (~77.34%) and 125 µg/mL for the hexane extract (~76.51%). The results suggest that Ghanaian shallots could potentially be useful when further developed to tackle antimicrobial resistance, particularly in tuberculosis (TB).

Item Type: Article
Uncontrolled Keywords: antimicrobial resistance,biofilms,efflux pumps,ht-spoti,tuberculosis,microbiology,biochemistry,pharmacology, toxicology and pharmaceutics(all),microbiology (medical),infectious diseases,pharmacology (medical) ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Science > School of Pharmacy
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Depositing User: LivePure Connector
Date Deposited: 27 Aug 2021 00:09
Last Modified: 01 Sep 2021 00:52
URI: https://ueaeprints.uea.ac.uk/id/eprint/81247
DOI: 10.3390/antibiotics10080902

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