Diversity, prevalence, and expression of cyanase genes (cynS) in planktonic marine microorganisms

Mao, Xuewei, Chen, Jianwei, van Oosterhout, Cock ORCID: https://orcid.org/0000-0002-5653-738X, Zhang, Huan, Liu, Guangxing, Zhuang, Yunyun and Mock, Thomas ORCID: https://orcid.org/0000-0001-9604-0362 (2022) Diversity, prevalence, and expression of cyanase genes (cynS) in planktonic marine microorganisms. The ISME Journal, 16 (2). 602–605. ISSN 1751-7362

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Cyanate is utilized by many microbes as an organic nitrogen source. The key enzyme for cyanate metabolism is cyanase, converting cyanate to ammonium and carbon dioxide. Although the cyanase gene cynS has been identified in many species, the diversity, prevalence, and expression of cynS in marine microbial communities remains poorly understood. Here, based on the full-length cDNA sequence of a dinoflagellate cynS and 260 homologs across the tree of life, we extend the conserved nature of cyanases by the identification of additional ultra-conserved residues as part of the modeled holoenzyme structure. Our phylogenetic analysis showed that horizontal gene transfer of cynS appears to be more prominent than previously reported for bacteria, archaea, chlorophytes, and metazoans. Quantitative analyses of marine planktonic metagenomes revealed that cynS is as prevalent as ureC (urease subunit alpha), suggesting that cyanate plays an important role in nitrogen metabolism of marine microbes. Highly abundant cynS transcripts from phytoplankton and nitrite-oxidizing bacteria identified in global ocean metatranscriptomes indicate that cyanases potentially occupy a key position in the marine nitrogen cycle by facilitating photosynthetic assimilation of organic N and its remineralisation to NO3 by the activity of nitrifying bacteria.

Item Type: Article
Additional Information: Funding Information: This study was supported by the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (2018SDKJ0406-3), National Natural Science Foundation of China (41876156), and Fundamental Research Funds for the Central Universities (201812019) to YZ and the China Scholarship Council (201806330141) to XM. TM and CvO acknowledge the School of Environmental Sciences at the University of East Anglia, Norwich, UK for partial support.
Uncontrolled Keywords: ecology, evolution, behavior and systematics,microbiology,sdg 14 - life below water ,/dk/atira/pure/subjectarea/asjc/1100/1105
Faculty \ School: Faculty of Science > School of Environmental Sciences
UEA Research Groups: Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences
Faculty of Science > Research Groups > Environmental Biology
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Depositing User: LivePure Connector
Date Deposited: 25 Aug 2021 09:54
Last Modified: 23 Oct 2022 02:56
URI: https://ueaeprints.uea.ac.uk/id/eprint/81203
DOI: 10.1038/s41396-021-01081-y

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