Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele

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Tomlinson, Laurence, Yang, Ying, Emenecker, Ryan, Smoker, Matthew, Taylor, Jodie, Perkins, Sara, Smith, Justine, MacLean, Dan, Olszewski, Neil E. and Jones, Jonathan D. G. (2019) Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele. Plant Biotechnology Journal, 17 (1). pp. 132-140. ISSN 1467-7644

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Abstract

The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.

Item Type:	Article
Uncontrolled Keywords:	cas9,della,dwarf,gibberellic acid,procera,slender,biotechnology,agronomy and crop science,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1305
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Computing Sciences Faculty of Science > School of Biological Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	28 Jul 2021 00:44
Last Modified:	09 Apr 2024 03:06
URI:	https://ueaeprints.uea.ac.uk/id/eprint/80865
DOI:	10.1111/pbi.12952

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