A new subspecies of Trypanosoma cyclops found in the Australian terrestrial leech Chtonobdella bilineata

Ellis, John, Barratt, Joel, Kauffer, Alexa, Pearn, Lauren, Armstrong, Brigette, Johnson, Michael, Park, Yasunori, Downey, Lara, Cao, Maisie, Neill, Levina, Lee, Rogan, Ellis, Bethany, Tyler, Kevin, Lun, Zhao-Rong and Stark, Damien (2021) A new subspecies of Trypanosoma cyclops found in the Australian terrestrial leech Chtonobdella bilineata. Parasitology. ISSN 0031-1820

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Abstract

Previously it was suggested that haemadipsid leeches represent an important vector of trypanosomes amongst native animals in Australia. Consequently, Chtonobdella bilineata leeches were investigated for the presence of trypanosome species by PCR, DNA sequencing, and in vitro isolation. Phylogenetic analysis ensued to further define the populations present. PCR targeting the 28S rDNA demonstrated that over 95% of C. bilineata contained trypanosomes; diversity profiling by deep amplicon sequencing of 18S rDNA indicated the presence of four different clusters related to the Trypanosoma (Megatrypanum) theileri. Novy-MacNeal-Nicolle (NNN) slopes with liquid overlay were used to isolate trypanosomes into culture that proved similar in morphology to Trypanosoma cyclops in that they contained a large numbers of acidocalcisomes. Phylogeny of 18S rDNA/GAPDH/ND5 DNA sequences from primary cultures and subclones showed the trypanosomes were monophyletic, with T. cyclops as a sister group. Blood meal analysis of leeches showed thatleeches primarily contained blood from Swamp Wallaby (Wallabia bicolour), human (Homo sapiens) or horse (Equus sp.). The leech C. bilineata is a host for at least five lineages of Trypanosoma sp. and these are monophyletic with T. cyclops; we propose Trypanosoma cyclops australiensis as a subspecies of T. cyclops based on genetic similarity and biogeography considerations.

Item Type: Article
Uncontrolled Keywords: diversity,leech,phylogeny,swamp wallaby,trypanosoma,trypanosoma cyclops,parasitology,animal science and zoology,infectious diseases ,/dk/atira/pure/subjectarea/asjc/2400/2405
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 08 May 2021 00:03
Last Modified: 14 Jun 2021 00:11
URI: https://ueaeprints.uea.ac.uk/id/eprint/79953
DOI: 10.1017/S0031182021000639

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