A new subspecies of Trypanosoma cyclops found in the Australian terrestrial leech Chtonobdella bilineata

Ellis, John, Barratt, Joel, Kauffer, Alexa, Pearn, Lauren, Armstrong, Brigette, Johnson, Michael, Park, Yasunori, Downey, Lara, Cao, Maisie, Neill, Levina, Lee, Rogan, Ellis, Bethany, Tyler, Kevin ORCID: https://orcid.org/0000-0002-0647-8158, Lun, Zhao-Rong and Stark, Damien (2021) A new subspecies of Trypanosoma cyclops found in the Australian terrestrial leech Chtonobdella bilineata. Parasitology, 148 (10). pp. 1125-1136. ISSN 0031-1820

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Previously, it was suggested that haemadipsid leeches represent an important vector of trypanosomes amongst native animals in Australia. Consequently, Chtonobdella bilineata leeches were investigated for the presence of trypanosome species by polymerase chain reaction (PCR), DNA sequencing and in vitro isolation. Phylogenetic analysis ensued to further define the populations present. PCR targeting the 28S rDNA demonstrated that over 95% of C. bilineata contained trypanosomes; diversity profiling by deep amplicon sequencing of 18S rDNA indicated the presence of four different clusters related to the Trypanosoma (Megatrypanum) theileri. Novy-MacNeal-Nicolle slopes with liquid overlay were used to isolate trypanosomes into culture that proved similar in morphology to Trypanosoma cyclops in that they contained a large numbers of acidocalcisomes. Phylogeny of 18S rDNA/GAPDH/ND5 DNA sequences from primary cultures and subclones showed the trypanosomes were monophyletic, with T. cyclops as a sister group. Blood-meal analysis of leeches showed that leeches primarily contained blood from swamp wallaby (Wallabia bicolour), human (Homo sapiens) or horse (Equus sp.). The leech C. bilineata is a host for at least five lineages of Trypanosoma sp. and these are monophyletic with T. cyclops; we propose Trypanosoma cyclops australiensis as a subspecies of T. cyclops based on genetic similarity and biogeography considerations.

Item Type: Article
Additional Information: Special Issue: Kinetoplastid cell biology and genetics // Sequence data availability: Sequences of the 18S rDNA, GAPDH and ND5 loci derived from the trypanosomes described in this study have been submitted to GenBank and can be found under accession numbers MW872344 to MW872359 and MW874225 to MW874260.
Uncontrolled Keywords: diversity,leech,phylogeny,swamp wallaby,trypanosoma,trypanosoma cyclops,leech,phylogeny,swamp wallaby,infectious diseases,animal science and zoology,parasitology,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2725
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
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Depositing User: LivePure Connector
Date Deposited: 08 May 2021 00:03
Last Modified: 19 Oct 2023 02:57
URI: https://ueaeprints.uea.ac.uk/id/eprint/79953
DOI: 10.1017/S0031182021000639


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