Understanding the potential in vitro modes of action of bis(β‐diketonato) oxovanadium(IV) complexes

Sergi, Baris, Bulut, Ipek, Xia, Ying, Waller, Zoë A. E., Yildizhan, Yasemin, Acilan, Ceyda and Lord, Rianne M. (2021) Understanding the potential in vitro modes of action of bis(β‐diketonato) oxovanadium(IV) complexes. ChemMedChem. ISSN 1860-7179

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Abstract

To understand the potential in vitro modes of action of bis(β-diketonato) oxovanadium(IV) complexes, nine compounds of varying functionality have been screened using a range of biological techniques. The antiproliferative activity against a range of cancerous and normal cell lines has been determined, and show these complexes are particularly sensitive against the lung carcinoma cell line, A549. Annexin V (apoptosis) and Caspase-3/7 assays were studied to confirm these complexes induce programmed cell death. While gel electrophoresis was used to determine DNA cleavage activity and production of reactive oxygen species (ROS), the Comet assay was used to determine induced genomic DNA damage. Additionally, Förster resonance energy transfer (FRET)-based DNA melting and fluorescent intercalation displacement assays have been used to determine the interaction of the complexes with double strand (DS) DNA and to establish preferential DNA base-pair binding (AT versus GC).

Item Type: Article
Uncontrolled Keywords: apoptosis,bioinorganic chemistry,dna interactions,vanadium complexes,β-diketonate complexes,biochemistry,molecular medicine,pharmacology,drug discovery,pharmacology, toxicology and pharmaceutics(all),organic chemistry ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School: Faculty of Science > School of Pharmacy
Faculty of Science > School of Chemistry
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 05 May 2021 00:03
Last Modified: 09 Jul 2021 07:22
URI: https://ueaeprints.uea.ac.uk/id/eprint/79923
DOI: 10.1002/cmdc.202100152

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