Non-polymeric nanogels as versatile nanocarriers: Intracellular transport of the photosensitizers Rose Bengal and Hypericin for photodynamic therapy

Torres-Martinez, Ana, Bedrina, Begoña, Falomir, Eva, Marin, Maria J., Angulo-Pachon, Cesar A., Galindo, Francisco and Miravet, Juan F. (2021) Non-polymeric nanogels as versatile nanocarriers: Intracellular transport of the photosensitizers Rose Bengal and Hypericin for photodynamic therapy. ACS Applied Bio Materials, 4 (4). 3658–3669. ISSN 2576-6422

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Abstract

The use of nanocarriers for intracellular transport of actives has been extensively studied in recent years and represents a central area of Nanomedicine. The main novelty of this paper lies on the use of nanogels formed by a low molecular weight gelator (1). Here, nonpolymeric, molecular nanogels are successfully used for intracellular transport of two photodynamic therapy (PDT) agents, Rose Bengal (RB) and Hypericin (HYP). The two photosensitizers (PSs) exhibit different drawbacks for their use in clinical applications. HYP is poorly water-soluble, while the cellular uptake of RB is hindered due to its dianionic character at physiological pH values. Additionally, both PSs tend to aggregate precluding an effective PDT. Despite the different nature of these PSs, nanogels from gelator 1 provide, in both cases, an efficient intracellular transport into human colon adenocarcinoma cells (HT-29) and a notably improved PDT efficiency, as assessed by confocal laser scanning microscopy and flow cytometry. Furthermore, no significant dark toxicity of the nanogels is observed, supporting the biocompatibility of the delivery system. The developed nanogels are highly reproducible due to their non-polymeric nature and their synthesis is easily scaled up. The results here presented confirm thus the potential of molecular nanogels as valuable nanocarriers, capable of entrapping both hydrophobic and hydrophilic actives, for PDT of cancer.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
Depositing User: LivePure Connector
Date Deposited: 13 Apr 2021 23:49
Last Modified: 10 May 2021 00:15
URI: https://ueaeprints.uea.ac.uk/id/eprint/79715
DOI: 10.1021/acsabm.1c00139

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