Identification of the vibrational marker of tyrosine cation radical using ultrafast transient infrared spectroscopy of flavoprotein systems

Pirisi, Katalin, Nag, Lipsa, Fekete, Zsuzsanna, Iuliano, James N., Tolentino Collado, Jinnette, Clark, Ian P., Pécsi, Ildikó, Sournia, Pierre, Liebl, Ursula, Greetham, Gregory M., Tonge, Peter J., Meech, Stephen R., Vos, Marten H. and Lukacs, Andras (2021) Identification of the vibrational marker of tyrosine cation radical using ultrafast transient infrared spectroscopy of flavoprotein systems. Photochemical & Photobiological Sciences, 20 (3). 369–378. ISSN 1474-905X

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Abstract

Tryptophan and tyrosine radical intermediates play crucial roles in many biological charge transfer processes. Particularly in flavoprotein photochemistry, short-lived reaction intermediates can be studied by the complementary techniques of ultrafast visible and infrared spectroscopy. The spectral properties of tryptophan radical are well established, and the formation of neutral tyrosine radicals has been observed in many biological processes. However, only recently, the formation of a cation tyrosine radical was observed by transient visible spectroscopy in a few systems. Here, we assigned the infrared vibrational markers of the cationic and neutral tyrosine radical at 1483 and 1502 cm−1 (in deuterated buffer), respectively, in a variant of the bacterial methyl transferase TrmFO, and in the native glucose oxidase. In addition, we studied a mutant of AppABLUF blue-light sensor domain from Rhodobacter sphaeroides in which only a direct formation of the neutral radical was observed. Our studies highlight the exquisite sensitivity of transient infrared spectroscopy to low concentrations of specific radicals.

Item Type: Article
Uncontrolled Keywords: physical and theoretical chemistry ,/dk/atira/pure/subjectarea/asjc/1600/1606
Faculty \ School: Faculty of Science > School of Chemistry
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Depositing User: LivePure Connector
Date Deposited: 26 Feb 2021 01:02
Last Modified: 30 Sep 2021 16:15
URI: https://ueaeprints.uea.ac.uk/id/eprint/79337
DOI: 10.1007/s43630-021-00024-y

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