Non‐canonical autophagy functions of ATG16L1 in epithelial cells limit lethal infection by influenza A virus

Wang, Yingxue, Sharma, Parul, Jefferson, Matthew, Zhang, Weijiao, Bone, Ben, Kipar, Anja, Bitto, David, Coombes, Janine L., Pearson, Timothy, Man, Angela, Zhekova, Alex, Bao, Yongping, Tripp, Ralph A., Carding, Simon R., Yamauchi, Yohei, Mayer, Ulrike, Powell, Penny P., Stewart, James P. and Wileman, Thomas (2021) Non‐canonical autophagy functions of ATG16L1 in epithelial cells limit lethal infection by influenza A virus. The EMBO Journal, 40 (6). ISSN 0261-4189

[img]
Preview
PDF (Published_Version) - Published Version
Available under License Creative Commons Attribution.

Download (3MB) | Preview

Abstract

Influenza A virus (IAV) and SARS-CoV-2 (COVID-19) cause pandemic infections where cytokine storm syndrome and lung inflammation lead to high mortality. Given the high social and economic cost of respiratory viruses, there is an urgent need to understand how the airways defend against virus infection. Here we use mice lacking the WD and linker domains of ATG16L1 to demonstrate that ATG16L1- dependent targeting of LC3 to single-membrane, non-autophagosome compartments – referred to as non-canonical autophagy – protects mice from lethal IAV infection. Mice with systemic loss of non-canonical autophagy are exquisitely sensitive to low-pathogenicity IAV where extensive viral replication throughout the lungs, coupled with cytokine amplification mediated by plasmacytoid dendritic cells, leads to fulminant pneumonia, lung inflammation and high mortality. IAV was controlled within epithelial barriers where non-canonical autophagy reduced IAV fusion with endosomes and activation of interferon signalling. Conditional mouse models and ex vivo analysis showed that protection against IAV infection of lung was independent of phagocytes and other leucocytes. This establishes non-canonical autophagy in airway epithelial cells as a novel innate defence that restricts IAV infection and lethal inflammation at respiratory surfaces.

Item Type: Article
Uncontrolled Keywords: atg16l1 wd domain,cytokine storm,influenza,intrinsic defence,non-canonical autophagy,neuroscience(all),molecular biology,biochemistry, genetics and molecular biology(all),immunology and microbiology(all) ,/dk/atira/pure/subjectarea/asjc/2800
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 19 Feb 2021 01:05
Last Modified: 02 Dec 2021 02:43
URI: https://ueaeprints.uea.ac.uk/id/eprint/79289
DOI: 10.15252/embj.2020105543

Actions (login required)

View Item View Item