Genomic diversity of Escherichia coli isolates from non-human primates in the Gambia

Foster-Nyarko, Ebenezer, Alikhan, Nabil-Fareed, Ravi, Anuradha, Thilliez, Gaëtan, Thomson, Nicholas M, Baker, David, Kay, Gemma, Cramer, Jennifer D, O'Grady, Justin, Antonio, Martin and Pallen, Mark J (2020) Genomic diversity of Escherichia coli isolates from non-human primates in the Gambia. Microbial Genomics, 6 (9). ISSN 2057-5858

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Abstract

Increasing contact between humans and non-human primates provides an opportunity for the transfer of potential pathogens or antimicrobial resistance between host species. We have investigated genomic diversity and antimicrobial resistance in Escherichia coli isolates from four species of non-human primates in the Gambia: Papio papio (n=22), Chlorocebus sabaeus (n=14), Piliocolobus badius (n=6) and Erythrocebus patas (n=1). We performed Illumina whole-genome sequencing on 101 isolates from 43 stools, followed by nanopore long-read sequencing on 11 isolates. We identified 43 sequence types (STs) by the Achtman scheme (ten of which are novel), spanning five of the eight known phylogroups of E. coli. The majority of simian isolates belong to phylogroup B2 - characterized by strains that cause human extraintestinal infections - and encode factors associated with extraintestinal disease. A subset of the B2 strains (ST73, ST681 and ST127) carry the pks genomic island, which encodes colibactin, a genotoxin associated with colorectal cancer. We found little antimicrobial resistance and only one example of multi-drug resistance among the simian isolates. Hierarchical clustering showed that simian isolates from ST442 and ST349 are closely related to isolates recovered from human clinical cases (differences in 50 and 7 alleles, respectively), suggesting recent exchange between the two host species. Conversely, simian isolates from ST73, ST681 and ST127 were distinct from human isolates, while five simian isolates belong to unique core-genome ST complexes - indicating novel diversity specific to the primate niche. Our results are of planetary health importance, considering the increasing contact between humans and wild non-human primates.

Item Type: Article
Uncontrolled Keywords: escherichia coli,extraintestinal pathogenic e. coli,non-human primates,phylogenomic diversity,epidemiology,microbiology,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2700/2713
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School

Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 09 Feb 2021 01:03
Last Modified: 12 Jun 2021 12:04
URI: https://ueaeprints.uea.ac.uk/id/eprint/79192
DOI: 10.1099/mgen.0.000428

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