The RNA polymerase II subunit RPB-9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription

Berkyurek, Ahmet C., Furlan, Giulia, Lampersberger, Lisa, Beltran, Toni, Weick, Eva-Maria, Nischwitz, Emily, Cunha Navarro, Isabela, Braukmann, Fabian, Akay, Alper, Price, Jonathan, Butter, Falk, Sarkies, Peter and Miska, Eric A. (2021) The RNA polymerase II subunit RPB-9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription. The EMBO Journal, 40 (5). ISSN 0261-4189

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Abstract

PIWI-interacting RNAs (piRNAs) are genome-encoded small RNAs that regulate germ cell development and maintain germline integrity in many animals. Mature piRNAs engage Piwi Argonaute proteins to silence complementary transcripts, including transposable elements and endogenous genes. piRNA biogenesis mechanisms are diverse and remain poorly understood. Here, we identify the RNA polymerase II (RNA Pol II) core subunit RPB-9 as required for piRNA-mediated silencing in the nematode Caenorhabditis elegans. We show that rpb-9 initiates heritable piRNA-mediated gene silencing at two DNA transposon families and at a subset of somatic genes in the germline. We provide genetic and biochemical evidence that RPB-9 is required for piRNA biogenesis by recruiting the Integrator complex at piRNA genes, hence promoting transcriptional termination. We conclude that, as a part of its rapid evolution, the piRNA pathway has co-opted an ancient machinery for high-fidelity transcription.

Item Type: Article
Uncontrolled Keywords: rna polymerase ii,integrator,pirnas,rpb-9,transcription termination,neuroscience(all),molecular biology,biochemistry, genetics and molecular biology(all),immunology and microbiology(all) ,/dk/atira/pure/subjectarea/asjc/2800
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 05 Feb 2021 02:58
Last Modified: 12 Jun 2021 12:03
URI: https://ueaeprints.uea.ac.uk/id/eprint/79168
DOI: 10.15252/embj.2020105565

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