Multiple holins contribute to extracellular DNA release in Pseudomonas aeruginosa biofilms

Hynen, Amelia L., Lazenby, James J., Savva, George M., McCaughey, Laura C., Turnbull, Lynne, Nolan, Laura M. and Whitchurch, Cynthia B. ORCID: (2021) Multiple holins contribute to extracellular DNA release in Pseudomonas aeruginosa biofilms. Microbiology, 167 (2). ISSN 1350-0872

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Bacterial biofilms are composed of aggregates of cells encased within a matrix of extracellular polymeric substances (EPS). One key EPS component is extracellular DNA (eDNA), which acts as a ‘glue’, facilitating cell–cell and cell–substratum interactions. We have previously demonstrated that eDNA is produced in Pseudomonas aeruginosa biofilms via explosive cell lysis. This phenomenon involves a subset of the bacterial population explosively lysing, due to peptidoglycan degradation by the endolysin Lys. Here we demonstrate that in P. aeruginosa three holins, AlpB, CidA and Hol, are involved in Lys-mediated eDNA release within both submerged (hydrated) and interstitial (actively expanding) biofilms, albeit to different extents, depending upon the type of biofilm and the stage of biofilm development. We also demonstrate that eDNA release events determine the sites at which cells begin to cluster to initiate microcolony formation during the early stages of submerged biofilm development. Furthermore, our results show that sustained release of eDNA is required for cell cluster consolidation and subsequent microcolony development in submerged biofilms. Overall, this study adds to our understanding of how eDNA release is controlled temporally and spatially within P. aeruginosa biofilms.

Item Type: Article
Uncontrolled Keywords: eps,biofilm initiation,explosive cell lysis,extracellular polymeric substance,microcolony formation,microbiology ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Medicine and Health Sciences > School of Health Sciences
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Norwich Epidemiology Centre
Faculty of Medicine and Health Sciences > Research Groups > Norwich Epidemiology Centre
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Depositing User: LivePure Connector
Date Deposited: 02 Feb 2021 01:03
Last Modified: 04 Dec 2023 02:17
DOI: 10.1099/mic.0.000990


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