Distinct modes of derepression of an Arabidopsis immune receptor complex by two different bacterial effectors

Ma, Yan, Guo, Hailong, Hu, Lanxi, Martinez, Paula Pons, Moschou, Panagiotis N., Cevik, Volkan, Ding, Pingtao, Duxbury, Zane, Sarris, Panagiotis F. and Jones, Jonathan D. G. (2018) Distinct modes of derepression of an Arabidopsis immune receptor complex by two different bacterial effectors. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 115 (41). pp. 10218-10227. ISSN 0027-8424

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Plant intracellular nucleotide-binding leucine-rich repeat (NLR) immune receptors often function in pairs to detect pathogen effectors and activate defense. The Arabidopsis RRS1-R–RPS4 NLR pair recognizes the bacterial effectors AvrRps4 and PopP2 via an integrated WRKY transcription factor domain in RRS1-R that mimics the effector’s authentic targets. How the complex activates defense upon effector recognition is unknown. Deletion of the WRKY domain results in an RRS1 allele that triggers constitutive RPS4-dependent defense activation, suggesting that in the absence of effector, the WRKY domain contributes to maintaining the complex in an inactive state. We show the WRKY domain interacts with the adjacent domain 4, and that the inactive state of RRS1 is maintained by WRKY–domain 4 interactions before ligand detection. AvrRps4 interaction with the WRKY domain disrupts WRKY–domain 4 association, thus derepressing the complex. PopP2-triggered activation is less easily explained by such disruption and involves the longer C-terminal extension of RRS1-R. Furthermore, some mutations in RPS4 and RRS1 compromise PopP2 but not AvrRps4 recognition, suggesting that AvrRps4 and PopP2 derepress the complex differently. Consistent with this, a “reversibly closed” conformation of RRS1-R, engineered in a method exploiting the high affinity of colicin E9 and Im9 domains, reversibly loses AvrRps4, but not PopP2 responsiveness. Following RRS1 derepression, interactions between domain 4 and the RPS4 C-terminal domain likely contribute to activation. Simultaneous relief of autoinhibition and activation may contribute to defense activation in many immune receptors.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > The Sainsbury Laboratory
UEA Research Groups: Faculty of Science > Research Groups > Plant Sciences
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Depositing User: LivePure Connector
Date Deposited: 30 Jan 2021 01:12
Last Modified: 22 Oct 2022 07:42
URI: https://ueaeprints.uea.ac.uk/id/eprint/78333
DOI: 10.1073/pnas.1811858115


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