Trypanosoma brucei transferrin receptor: functional replacement of the GPI anchor with a transmembrane domain

Kabiri, Mostafa and Steverding, Dietmar (2021) Trypanosoma brucei transferrin receptor: functional replacement of the GPI anchor with a transmembrane domain. Molecular and Biochemical Parasitology, 242. ISSN 0166-6851

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Abstract

The transferrin receptor of Trypanosoma brucei (TbTfR) is a heterodimer of a glycosylphosphatidylinositol (GPI)-anchored ESAG6 subunit and an ESAG7 subunit. To investigate whether the GPI-anchor is essential for the function of the TbTfR, an ESAG6 with a transmembrane domain instead of a GPI-anchor (ESAG6tmd) was inducibly expressed in bloodstream form trypanosomes. It is shown that the ESAG6tmd is able to dimerise with ESAG7 to form a TbTfR that can bind transferrin. Fractionation experiments clearly demonstrated that the transmembrane-anchored TbTfR is exclusively associated with the membrane fraction. No difference in the uptake of transferrin was observed between trypanosomes inducibly expressing a transmembrane-anchored TbTfR and trypanosomes inducibly expressing a GPI-anchored TbTfR. Differences in glycosylation pattern of ESAG6tmd and native ESAG6 may indicate different intracellular trafficking of transmembrane- and GPI-anchored TbTfRs. The findings suggest that the GPI-anchor is not essential for the function of the TbTfR in bloodstream forms of T. brucei.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 14 Jan 2021 00:54
Last Modified: 30 Sep 2021 16:02
URI: https://ueaeprints.uea.ac.uk/id/eprint/78178
DOI: 10.1016/j.molbiopara.2021.111361

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