Cleavage of a pathogen apoplastic protein by plant subtilases activates host immunity

Wang, Shuaishuai, Xing, Rongkang, Wang, Yan, Shu, Haidong, Fu, Shenggui, Huang, Jie, Paulus, Judith K., Schuster, Mariana, Saunders, Diane G. O., Win, Joe, Vleeshouwers, Vivianne, Wang, Yuanchao, Zheng, Xiaobo, van der Hoorn, Renier A. l. and Dong, Suomeng (2021) Cleavage of a pathogen apoplastic protein by plant subtilases activates host immunity. New Phytologist, 229 (6). pp. 3424-3439. ISSN 0028-646X

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Abstract

The plant apoplast is a harsh environment in which hydrolytic enzymes, especially proteases, accumulate during pathogen infection. However, the defense functions of most apoplastic proteases remain largely elusive. We show that a newly identified small cysteine rich secreted protein PC2 from the potato late blight pathogen Phytophthora infestans induces immunity in Solanum plants only after cleavage by plant apoplastic subtilisin‐like proteases, such as tomato P69B. A minimal 61‐amino‐acid core peptide carrying two key cysteines, conserved widely in most oomycete species, is sufficient for PC2‐induced cell death. Furthermore, we showed that Kazal‐like protease inhibitors, such as EPI1 produced by P. infestans prevent PC2 cleavage and dampen PC2 elicited host immunity. This study reveals that cleavage of pathogen proteins to release immunogenic peptides is an important function of plant apoplastic proteases.

Item Type: Article
Uncontrolled Keywords: p69b,pc2,phytophthora,apoplast,cleavage,immunity,serine protease,small cysteine-rich effector,physiology,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 03 Dec 2020 00:49
Last Modified: 12 Jun 2021 11:55
URI: https://ueaeprints.uea.ac.uk/id/eprint/77883
DOI: 10.1111/nph.17120

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