Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain

Serramito-Gómez, Inmaculada, Boada-Romero, Emilio, Villamuera, Raquel, Fernández-Cabrera, Álvaro, Cedillo, José Luis, Martín-Regalado, Ángela, Carding, Simon, Mayer, Uli, Powell, Penny P., Wileman, Thomas, García-Higuera, Irene and Pimentel-Muiños, Felipe X. (2020) Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain. Nature Communications, 11 (1). ISSN 2041-1723

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Abstract

ATG16L1, an autophagy mediator that specifies the site of LC3 lipidation, includes a C-terminal domain formed by 7 WD40-type repeats (WD40 domain, WDD), the function of which is unclear. Here we show that the WDD interacts with the intracellular domain of cytokine receptors to regulate their signaling output in response to ligand stimulation. Using a refined version of a previously described WDD-binding amino acid motif, here we show that this element is present in the intracellular domain of cytokine receptors. Two of these receptors, IL-10RB and IL-2Rγ, recognize the WDD through the motif and exhibit WDD-dependent LC3 lipidation activity. IL-10 promotes IL-10RB/ATG16L1 interaction through the WDD, and IL-10 signaling is suboptimal in cells lacking the WDD owing to delayed endocytosis and inefficient early trafficking of IL10/IL-10R complexes. Our data reveal WDD-dependent roles of ATG16L1 in the regulation of cytokine receptor trafficking and signaling, and provide a WDD-binding motif that might be used to identify additional WDD activators.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 28 Nov 2020 00:50
Last Modified: 30 Sep 2021 15:55
URI: https://ueaeprints.uea.ac.uk/id/eprint/77827
DOI: 10.1038/s41467-020-19670-4

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