In silico peptide-directed ligand design complements experimental peptide-directed binding for protein–protein interaction modulator discovery
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ToolsHowell, Lesley and Beekman, Andrew (2020) In silico peptide-directed ligand design complements experimental peptide-directed binding for protein–protein interaction modulator discovery. RSC Chemical Biology. ISSN 2633-0679
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Abstract
Using the protein–protein interaction of Mcl-1/Noxa, two methods for efficient modulator discovery are directly compared. In silico peptide-directed ligand design is evaluated against experimental peptide-directed binding, allowing for the discovery of two new inhibitors of Mcl-1/Noxa with cellular activity. In silico peptide-directed ligand design demonstrates an in vitro hit rate of 80% (IC50 < 100 μM). The two rapid and efficient methods demonstrate complementary features for protein–protein interaction modulator discovery.
| Item Type: | Article |
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| Faculty \ School: | Faculty of Science > School of Pharmacy |
| Depositing User: | LivePure Connector |
| Date Deposited: | 20 Nov 2020 00:53 |
| Last Modified: | 21 Jan 2021 00:54 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/77738 |
| DOI: | 10.1039/D0CB00148A |
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