G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Macaulay, Iain C., Haerty, Wilfried, Kumar, Parveen, Li, Yang I., Hu, Tim Xiaoming, Teng, Mabel J., Goolam, Mubeen, Saurat, Nathalie, Coupland, Paul, Shirley, Lesley M., Smith, Miriam, Van Der Aa, Niels, Banerjee, Ruby, Ellis, Peter D., Quail, Michael A., Swerdlow, Harold P., Zernicka-Goetz, Magdalena, Livesey, Frederick J., Ponting, Chris P. and Voet, Thierry (2015) G&T-seq: parallel sequencing of single-cell genomes and transcriptomes. Nature Methods, 12 (6). pp. 519-522. ISSN 1548-7091

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The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 10 Nov 2020 01:17
Last Modified: 17 Nov 2020 01:10
URI: https://ueaeprints.uea.ac.uk/id/eprint/77608
DOI: 10.1038/nmeth.3370

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