Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

Angermueller, Christof, Clark, Stephen J., Lee, Heather J., Macaulay, Iain C., Teng, Mabel J., Hu, Tim Xiaoming, Krueger, Felix, Smallwood, Sébastien A., Ponting, Chris P., Voet, Thierry, Kelsey, Gavin, Stegle, Oliver and Reik, Wolf (2016) Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity. Nature Methods, 13 (3). pp. 229-232. ISSN 1548-7091

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Abstract

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 10 Nov 2020 01:17
Last Modified: 21 Jul 2021 01:41
URI: https://ueaeprints.uea.ac.uk/id/eprint/77606
DOI: 10.1038/nmeth.3728

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