Bacterial sensors define intracellular free energies for correct enzyme metalation

Osman, Deenah, Martini, Maria Alessandra, Foster, Andrew W., Chen, Junjun, Scott, Andrew J. P., Morton, Richard J., Steed, Jonathan W., Lurie-Luke, Elena, Huggins, Thomas G., Lawrence, Andrew D., Deery, Evelyne, Warren, Martin J., Chivers, Peter T. and Robinson, Nigel J. (2019) Bacterial sensors define intracellular free energies for correct enzyme metalation. Nature Chemical Biology, 15 (3). pp. 241-249. ISSN 1552-4450

Full text not available from this repository. (Request a copy)

Abstract

There is a challenge for metalloenzymes to acquire their correct metals because some inorganic elements form more stable complexes with proteins than do others. These preferences can be overcome provided some metals are more available than others. However, while the total amount of cellular metal can be readily measured, the available levels of each metal have been more difficult to define. Metal-sensing transcriptional regulators are tuned to the intracellular availabilities of their cognate ions. Here we have determined the standard free energy for metal complex formation to which each sensor, in a set of bacterial metal sensors, is attuned: the less competitive the metal, the less favorable the free energy and hence the greater availability to which the cognate allosteric mechanism is tuned. Comparing these free energies with values derived from the metal affinities of a metalloprotein reveals the mechanism of correct metalation exemplified here by a cobalt chelatase for vitamin B12.

Item Type: Article
Faculty \ School: Faculty of Science
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 24 Sep 2020 00:04
Last Modified: 19 Oct 2020 23:54
URI: https://ueaeprints.uea.ac.uk/id/eprint/76991
DOI: 10.1038/s41589-018-0211-4

Actions (login required)

View Item View Item