Evaluation of five chromogenic agar media and the Rosco Rapid Carb screen kit for detection and confirmation of carbapenemase production in Gram-negative bacilli

Simner, Patricia J, Gilmour, Matthew W, DeGagne, Pat, Nichol, Kim and Karlowsky, James A (2015) Evaluation of five chromogenic agar media and the Rosco Rapid Carb screen kit for detection and confirmation of carbapenemase production in Gram-negative bacilli. Journal of Clinical Microbiology, 53 (1). pp. 105-112. ISSN 0095-1137

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Abstract

An efficient workflow to screen for and confirm the presence of carbapenemase-producing Gram-negative bacilli was developed by evaluating five chromogenic screening agar media and two confirmatory assays, the Rapid Carb screen test (Rosco Diagnostica A/S, Taastrup, Denmark) and the modified Hodge test. A panel of 150 isolates was used, including 49 carbapenemase-producing isolates representing a variety of β-lactamase enzyme classes. An evaluation of analytical performance, assay cost, and turnaround time indicated that the preferred workflow (screening test followed by confirmatory testing) was the chromID Carba agar medium (bioMérieux, Marcy l'Étoile, France), followed by the Rapid Carb screen test, yielding a combined sensitivity of 89.8% and a specificity of 100%. As an optional component of the workflow, a determination of carbapenemase gene class via molecular means could be performed subsequent to confirmatory testing.

Item Type: Article
Additional Information: Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Uncontrolled Keywords: pharmacology,genetics,culture media,classification,diagnosis,humans,methods,multiplex polymerase chain reaction,reagent kits, diagnostic,reproducibility of results,workflow,genetics
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: LivePure Connector
Date Deposited: 28 Aug 2020 00:05
Last Modified: 16 Sep 2020 23:56
URI: https://ueaeprints.uea.ac.uk/id/eprint/76694
DOI: 10.1128/JCM.02068-14

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